Log In Sign up
The largest database of trusted experimental protocols

P nitrophenyl β glucopyranoside pnpg

Manufactured by Merck Group
Visit Supplier
Sourced in France, Germany

P-nitrophenyl-β-glucopyranoside (pNPG) is a colorimetric substrate used in biochemical assays. It is a synthetic, water-soluble compound that can be enzymatically hydrolyzed, generating a yellow-colored product that can be detected spectrophotometrically.

Automatically generated - may contain errors

Lab products found in correlation

Starch, by Merck Group (1 mentions) Porcine pancreatic α amylase type 6 b, by Merck Group (1 mentions) Type 1 α glucosidase from baker s yeast, by Merck Group (1 mentions) Vanillin, by Merck Group (1 mentions)

Close spelling variants of this product

P-nitrophenyl-β-D-glucopyranoside (pNPG; (29 citations) P-nitrophenyl glucopyranoside (pNPG) (11 citations) P-nitrophenyl glucopyranoside (16 citations)

2 protocols using p nitrophenyl β glucopyranoside pnpg

1

Enzymatic Starch Hydrolysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Culture media were purchased from Biokar Diagnostics (Solabia group, Pantin, France) and Conda Laboratories (Madrid, Spain). Reagents were obtained from Sigma-Aldrich (Saint-Quentin Fallavier, France) and VWR chemicals (Fontenay-sous-Bois, France). Type VI-B porcine pancreatic α-amylase, type I α-glucosidase from baker’s yeast, starch, p-nitrophenyl-β-glucopyranoside (pNPG) and voglibase and other chemicals came from Sigma-Aldrich (Saint-Quentin Fallavier, France).
Mashitoa F.M., Akinola S.A., Manhevi V.E., Garcia C., Remize F., Slabbert R.M, & Sivakumar D. (2021). Influence of Fermentation of Pasteurised Papaya Puree with Different Lactic Acid Bacterial Strains on Quality and Bioaccessibility of Phenolic Compounds during In Vitro Digestion. Foods, 10(5), 962.
+ Open protocol
+ Expand
2

Colorimetric Assay for Glucosidase Activity

Check if the same lab product or an alternative is used in the 5 most similar protocols
The p-nitrophenyl-β-glucopyranoside (pNPG) and vanillin were obtained from Sigma-Aldrich Co., Ltd. (Darmstadt, HE, Germany). Other chemicals and reagents were obtained from J.T. Baker® (Waltham, MA, USA).
Antonio-Gutiérrez O., Pacheco-Reyes I., Lagunez-Rivera L., Solano R., Cañizares-Macías M.D, & Vilarem G. (2023). Effect of Microwave and Ultrasound during the Killing Stage of the Curing Process of Vanilla (Vanilla planifolia, Andrews) Pods. Foods, 12(3), 469.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!