Beh c18 2.1 50 mm 1 7 μm column

Manufactured by Waters Corporation

The BEH C18 2.1 × 50 mm (1.7 μm) column is a liquid chromatography column manufactured by Waters Corporation. It features a 2.1 mm internal diameter, a length of 50 mm, and a particle size of 1.7 micrometers. The column is designed for use in high-performance liquid chromatography (HPLC) and ultra-high-performance liquid chromatography (UHPLC) applications.

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Lab products found in correlation

Xevo tq ms, by Waters Corporation (1 mentions) Acquity uplc system, by Waters Corporation (1 mentions) Heraeus megafuge 8r, by Thermo Fisher Scientific (1 mentions) 1290 lc system, by Waters Corporation (1 mentions) Qtrap 6500, by AB Sciex (1 mentions)

3 protocols using beh c18 2.1 50 mm 1 7 μm column

Quantitative Analysis of Compounds via LC-MS

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A Waters Xevo
TQ MS with electrospray
ionization was used to measure compound concentration. The mass spectrometer
was coupled to an Acquity UPLC system (Waters, Milford, MA) equipped
with a Waters BEH C18 2.1 × 50 mm (1.7 μm) column at 60
°C and an autosampler at 10 °C. The injection volume was
10 μL. The mobile phase was composed of two solvents: solvent
A, 5% acetonitrile and 0.1% formic acid in water; and solvent B, 0.1%
formic acid in acetonitrile. The chromatographic run consisted of
a linear gradient at a flow rate of 0.5 mL/min. The gradient comprised
an increase from 5% to 90% of solvent B from 0.5 to 1.2 min, followed
by a hold from 1.2 to 1.6 min and a return to the initial conditions
at 1.7 min until the end of the run (2 min). Mass transitions and
their respective cone voltages and collision energies are in Table
S1, Supporting Information.

Mateus A., Matsson P, & Artursson P. (2014). A High-Throughput Cell-Based Method to Predict the Unbound Drug Fraction in the Brain. Journal of Medicinal Chemistry, 57(7), 3005-3010.

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Quantification of Antiepileptic Drugs by HPLC

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Cell pellets were harvested 48 h after transfection and were washed in PBS. Then, the cells were lysed in methanol and centrifuged at 4°C to obtain the supernatant. The concentration of AEDs was measured by high-performance liquid chromatography (HPLC) with UV detection as described earlier (Shao et al., 2016 (link)). In this study, a Waters BEH-C18 (2.1 × 50 mm 1.7 μm) column was used. The mobile phase contained the following: A: water with 0.1% formic acid and B: acetonitrile. The flow rate was 0.4 ml/min, and a gradient was used as follows: 99% A: 1% B (initial), 40% A: 60% B (2 min), 5% A: 95% B (2.1 min), 5% A: 95% B (3.1 min), 99% A: 1% B (3.2 min), and 99% A: 1% B (4.5 min). The column oven temperature was set to 45°C. The chromatography conditions were as follows: negative ESI modes, curtain gas at 45.0 psi, ion spray voltage at 4500 V, and a temperature of 550°C; both ion source gases 1 and 2 were at 45.0 psi, DP-100, EP-10, and CXP-15. The intracellular levels of PB and PHT in the supernatant were quantified by performing a comparison against a standard curve derived from a standard solution of AEDs.

Xie Y., Wang M., Shao Y., Deng X, & Chen Y. (2019). Long Non-coding RNA KCNQ1OT1 Contributes to Antiepileptic Drug Resistance Through the miR-138-5p/ABCB1 Axis in vitro. Frontiers in Neuroscience, 13, 1358.

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Investigating Fenofibrate Release from MMC

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A shift in the UV spectrum occurred during the release of fenofibrate-loaded MMC, so UPLC-MS was used to determine the origin of the shift. Two samples were weighed into Eppendorf tubes, one with 8 mg fenofibrate-loaded MMC and the other with 0.7 mg crystalline fenofibrate and 7.2 mg MMC. Two milliliters of Milli-Q water was added to the tubes, which were then placed on a plate shaker at 37 °C for 24 h. The supernatants were recovered by centrifugation at 37 °C for 15 min at 23,000×g (Heraeus Megafuge 8R, ThermoScientific) and analyzed by obtaining a full MS spectrum (QTRAP 6500, AB Sciex). A stock solution of fenofibrate dissolved in DMSO was used as reference. Separation was performed on an Agilent 1290 LC-system, with a Waters BEH C18 2.1 × 50 mm (1.7 μm) column, and the run was made in positive mode. The mobile phase consisted of (A) 5% acetonitrile, 0.1% formic acid, and 94.9% Milli-Q water; and (B) 95% acetonitrile, 0.1% formic acid, and 4.9% Milli-Q water. A constant flow rate of 0.5 mL/ min was used for the gradient elution as follows: A was constant at 99% for 1.0 min, then decreased linearly to 20% for 7.30 min. A was thereafter decreased to 10% for 0.2 min, kept constant for 0.5 min, and then increased to 99% for 0.5 min.

Alvebratt C., Cheung O., Strømme M, & Bergström C.A.S. (2018). A Modified In Situ Method to Determine Release from a Complex Drug Carrier in Particle-Rich Suspensions. AAPS PharmSciTech, 19(7).

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