Three mice per treatment group underwent I/R injury by CAO for 60 min followed by reperfusion for another 24 h. Recently described [33 ] ROS-sensitive nanoparticles (5 μg/g BW) were intravenously injected 20 min before mice were euthanized and perfused with PBS; the hearts and blood were then collected. Each heart was cut into four transverse sections and imaged from both sides using an IVIS Lumina XRMS system (PerkinElmer). The 2D scans were performed using the following settings: filter passband = excitation at 420 nm and emission at 670 nm. Results are expressed as total radiance [p/s]/[μW/cm2] levels per g of tissue/blood. Cardiac sections were stained with 1% triphenyltetrazolium chloride (TTC) for 10 min at 37 °C in darkness and scanned using a high-resolution scanner (Epson Perfection Photo Scanner V370) to detect the infarct areas.
Ivis lumina xrms system
Manufactured by PerkinElmer
Sourced in United States
The IVIS Lumina XRMS system is a bioluminescence and fluorescence imaging platform from PerkinElmer. It enables the detection and quantification of light-emitting reporters in small animal models. The system provides high-sensitivity, low-noise imaging capabilities for a variety of applications.
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Lab products found in correlation
7 protocols using ivis lumina xrms system
1
ROS-sensitive Nanoparticles for Cardiac I/R Injury
2
Ex-vivo Murine and Human Bone Cultures for Breast Cancer Research
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Ex-vivo murine bone cultures were established with femurs from C57BL/6 female mice (EO771 cells) or NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG; #005557, Jackson’s Lab) female mice (MDA-MB-231). We 1) injected 105 EO771 breast cancer cells on femoral bones or 2) treated femurs with 50% CM from breast cancer cells in the presence/absence of Dasatinib (400 nM) and Quercetin (100 μM) for up to 5 days. Senolytics were added on day 0 and refreshed on day 3. Ex vivo human bone organ cultures were established with human cancellous bone fragments similar in size obtained from the femoral head discarded after hip arthroplasty. The bone samples were obtained from 2 females with no pathologies or medications that could affect bone mass or architecture. For these ex vivo cultures, we 1) plated 2×105 MDA-MB231 breast cancer cells on human bones or 2) treated them with 50% MDA-MB-231 CM for five days. The CM was refreshed every 72 hours. Tumor bioluminescence was imaged 10 min after incubating bones with D-luciferin (150 μg/ml) or coelenterazine (100 μM) using an IVIS lumina XRMS system (Perkin Elmer, MA, US).
3
Whole-Body and Ex Vivo Imaging of Fluorescent B. uniformis in Mice
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For whole-body intravital imaging of fluorescent B. uniformis, mice underwent general anesthesia with vaporized isoflurane (2.5% liter/min) provided in air anesthesia ports. Optical overlays of fluorescence signals on photographic images of mice were acquired with the IVIS imaging platform (IVIS Lumina XRMS system, PerkinElmer). Workflows for imaging acquisition and analysis implemented in the software imaging wizard (Living Image Software, PerkinElmer) followed three steps: setting excitation and emission filters for 570 and 520 nm, respectively; adjusting Field of View (FOV)-E; and manually setting exposure time to 5 s. For Ex vivo imaging, intestines dissected from mice were immediately transferred into PBS at 4°C and gently trimmed of fat tissue, mesentery lymph nodes, and blood from the intestinal surface. The intestines were then placed into the imaging chamber with FOV-D setting adjustment. Luminescent exposure time was set to 1.2 s. All other setting parameters were left unchanged.
4
In Vivo Bioluminescence Imaging of Mice
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Seven-day-old Kunming mice were infected by r611-iLOV or r611-Nluc via the oral route. All fluorescence or bioluminescence data were collected using an IVIS Lumina XRMS system (PerkinElmer, USA). All mice were anesthetized via isoflurane inhalation (5% isoflurane, oxygen flow rate of 1.5 L/min) prior to and during bioluminescence imaging (BLI) using the gas anesthesia system (Matrix, Newton, MA, USA). The nano-Glo substrate (Promega, Fitchburg, WI, USA) was diluted 1:20 in PBS. Each mouse in the groups was i.p. injected with 100 μL of the mixture 5–8 min before imaging. The mice were imaged in both dorsal and ventral views at days 1, 3 and 6 post infection. Images were acquired and analyzed with living image in vivo software (PerkinElmer, USA). Photon flux was measured as luminescent radiance (p/sec/cm2/sr).
5
Targeted Cell Tracking in Myocardial Infarction
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PBMCs were stained using 25 µM CellTrackerTM Red CMTPX Dye (Molecular Probes, excitation (Ex), 577 nm; emission (Em), 602 nm) for 30 min at 37 °C. Cells were then washed twice in DMEM and incubated with 140 µg/ml Tand-scFvSca-1+GPIIb/IIIa or Tand-scFvSca-1+Mutant for 15 min at RT. Mice underwent MI-inducing surgery by CAO. Immediately after reperfusion, cells were injected and mice were euthanized either 3 h or 24 h after reperfusion. The organs were perfused with PBS, collected in 10% formalin, and imaged using an IVIS Lumina XRMS system (Perkin Elmer).
After the IVIS scan the hearts were embedded in paraffin, cut into 6 µm thick slides and stained with 4',6-diamidino-2-phenylindole (DAPI). Samples were imaged using the Nikon A1r Plus Confocal Microscope, 40x objective.
After the IVIS scan the hearts were embedded in paraffin, cut into 6 µm thick slides and stained with 4',6-diamidino-2-phenylindole (DAPI). Samples were imaged using the Nikon A1r Plus Confocal Microscope, 40x objective.
6
In Vivo Imaging of Kidney Infection
7
Evaluating Compound 62520 Against A. baumannii Infection
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Eight-week-old female BALB/c mice were maintained under specific pathogen-free conditions. Mice were intraperitoneally infected with 1.0 × 109 CFUs of A. baumannii ATCC 17978-lux strain [24 (link)]. Subsequently, 2 h after bacterial infection, compound 62520 (2.8 mg/kg in 100 μL PBS) was intraperitoneally injected in the opposite side of their abdomen. The control mice were injected with the same volume of PBS. The infected mice were subjected to bioluminescence analysis using the IVIS Lumina XRMS system (Perkin Elmer, Waltham, MA, USA). Images were captured at the indicated time points (3 and 24 h post-infection), and luminescence as radiance (p/sec/cm2/sr) was acquired by a computer using Living Image Software (Perkin Elmer). All animal experiments were performed in accordance with regulations established by the Institutional Animal Care and Use Committee of Kyungpook National University.
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