Serum-free and serum-containing cultures were plated in DMEM supplemented with B27 (Invitrogen Life Technologies) and 10% FBS/DMEM. Cell proliferation assay was determined by cell counting kit CCK-8 assay (Dojindo Laboratories, Kumamoto, Japan) at optical density 450 nm using the Victor spectrophotometer (PerkinElmer Life Sciences, MA, USA). For the Tet-inducible growth kinetic assay, U251-E6 and U251-E18 cells were seeded in a 96-well plate at a density of at 5000 cells per well. After 24 h, cells were either assayed at Day 1 (without Tet) for cell viability or cultured in the presence or absence of 1 µg/mL Tet (Sigma-Aldrich) diluted in serum-free DMEM. Subsequently, the cell viability of these cells was assayed at Day 2 and Day 4. For AG1478 treatment, glioma cell lines and primary glioma cells were cultured under serum-free condition. The cells were incubated, with or without AG1478 (10 µM, Sigma-Aldrich) for 24 h prior to CCK-8 assay or western blot analysis.
Victor spectrophotometer
Manufactured by PerkinElmer
Sourced in United States
The Victor spectrophotometer is a high-performance laboratory instrument designed for absorbance and fluorescence measurements. It offers precise and sensitive detection capabilities for a wide range of applications in life science research and clinical diagnostics.
Automatically generated - may contain errors
Lab products found in correlation
Cck 8 assay, by Dojindo Laboratories (3 mentions)
Ag1478, by Merck Group (1 mentions)
Mpo fluorometric activity assay kit, by Merck Group (1 mentions)
Bgj398, by Selleck Chemicals (1 mentions)
Norepinephrine ne, by Merck Group (1 mentions)
Luciferin, by PerkinElmer (1 mentions)
Cell proliferation kit 2 xtt, by Roche (1 mentions)
Cell proliferation kit 2, by Roche (1 mentions)
Zeba spin desalting column, by Thermo Fisher Scientific (1 mentions)
Eclipse ti inverted microscope, by Nikon (1 mentions)
14 protocols using victor spectrophotometer
1
Comparative Glioma Cell Proliferation
2
Quantification of Neutrophil MPO Activity
3
Norepinephrine and BGJ398 Cell Assay
4
Extracellular ROS Production Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Extracellular ROS production was measured in triplicate by the superoxide dismutase (SOD)-inhibitable reduction of cytochrome c (absorbance at 550 nm). Cells (5×106 cells/well) cultured on 12-well plates were incubated in 0.5 ml phenol-free DMEM/F12 medium containing reduced cytochrome c (200 μmol/L) with or without SOD (200 μmol/L) for 15 min at 37 °C. The incubation was continued for another 30 min in the presence of vehicle or the D1-like receptor agonist, fenoldopam (1 µmol/L), and the absorbance of reduced cytochrome c was measured at 550 nm optical density (OD), using a Victor spectrophotometer (PerkinElmer Life, Shelton, CT). SOD-inhibitable reduction of cytochrome c was calculated by the formula [nmol=159×(OD−SOD−OD+SOD)] [37] (link), [38] (link). The results were expressed as nmol/5×106 cells. To compare vehicle and drug treatment groups, the percentage of the absolute value of each drug treatment relative to the absolute mean of the vehicle-treated controls was calculated.
5
HaCaT Cell Viability Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HaCaT cells were plated into 96-well plates at 2 × 104 cells/well. Cells were then starved for 24 hours in DMEM high glucose medium supplemented with 0.5% serum and were treated with plant extracts. After 24 hours, cell viability was assessed using the XTT cell proliferation kit II (number 11465015001, Roche, Basel, Switzerland) according to the manufacturer’s instructions. Cells were incubated with the XTT solution at a final concentration of 0.3 mg/ml for 4 hours at 37 °C, and the absorbance of formazan product was measured at 470 nm using a Victor spectrophotometer (PerkinElmer).
6
RAD51C Mutant Sensitivity Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The parental V79B and CL-V4B stably expressed RAD51C mutants were treated with inter-strand crosslink (ICLs) agent MMC, Etoposide, PARP inhibitor and HU to assess cellular viability. Cells seeded onto 96-well plate at a density of at 5000 cells per well were treated with Mitomycin C (100 nM, 5 days), Etoposide (10 nM for 6 hours and recovered with fresh media for 4 days) Talazoparib (1 μM, 5 Days) and Hydroxyurea (0.4 mM for 12 hours and recovered with fresh media for 4 days). Cell viability was determined via the cell counting kit CCK-8 assay (Dojindo Laboratories, Kumamoto, Japan) at an optical density of 450 nm on the Victor spectrophotometer (PerkinElmer Life Sciences, Waltham,MA).
7
Evaluating Glioma Cell Viability
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell viability was determined by cell counting kit-8 (CCK-8) assay (Dojindo, Japan), which measures the number of viable cells based on bioreduction of a water soluble formazan. Human glioma cells (5000 cells/well) were seeded in 96-well dish and 24 h later, Nimotuzumab, rapamycin and respective controls were added to the cells. After an additional 24 h of respective treatments, 10% (v/v) of CCK-8 dye was added into the wells and cells were incubated for 1-2 h. The percentage of viable cells was then determined by measuring absorbance at an optical density (OD) 450 nm with a reference at 650 nm using Victor spectrophotometer (PerkinElmer Life Sciences, Waltham, MA). The percentage cell viability for each cell line and treatment group was normalized to respective vehicle controls.
8
Quantification of Inflammatory Cytokines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
IL-10, TNF-α, GM-CSF, and IL-8 were measured using ELISA kits (R&D systems, Minneapolis, MN, USA) according to the manufacturer´s protocol. Optical absorbance at 450 nm was measured by a Victor Spectrophotometer (PerkinElmer, Waltham, MA, USA).
9
Quantifying Cell Metabolic Activity
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell Proliferation kit II (XTT- Roche) allows to evaluate the cell metabolic activity by assessing the activity of intracellular oxydoreductases. XTT is a colorless or slightly yellow compound that becomes brightly orange upon reduction by cellular effectors as mitochondrial oxidoreductases. HaCaT cells were seeded in 24 well plates (2.5 × 105 per well) and treated as indicated. XTT assay was performed according to manufacturer’s instructions. Briefly, XTT solution at a final concentration 0.3 mg/mL was added in each well and the incubation was carried out for 4 h at 37 °C. During this incubation, the conversion of the yellow tetrazolium salt XTT by viable cells led to the formation of orange formazan solution. The intensity of coloration was quantified by measuring the absorbance (450 nm) using Victor spectrophotometer (PerkinElmer, Waltham, MA, USA).
10
Tubulin Polymerization Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The polymerisation of purified bovine brain tubulin (99% purity) was monitored using the Tubulin Polymerization Assay Kit from CytoDYNAMIX (Screen 03, Cytoskeleton Inc., Denver, CO, USA; Shelanski et al, 1973 (link); Lee and Timasheff, 1977 (link)). Briefly, tubulin polymerisation driven by guanosine triphosphate (GTP) and paclitaxel was monitored spectrophotometrically by the change in absorbance at 350 nm. The absorbance was measured at 1-min intervals for 60 min using a Victor spectrophotometer (Perkin-Elmer, Buckinghamshire, UK).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!