The following compounds were purchased from FUJIFILM Wako Pure Chemical Co. (Osaka, Japan): [6]-gingerol (Lot. APQ6161), amygdalin (Lot. KPM0958), glycyrrhizin (Lot. CAH4202), berberine (Lot. PTM0089), and (Z)-ligustilide (Lot. ESJ5922). The solvents used for HPLC and TLC analyses were of research grade. The TLC plate (Silica Gel 60 F254) was purchased from Merck Co. (Darmstadt, Germany).
6 gingerol
Manufactured by Fujifilm
Sourced in Japan
6-gingerol is a naturally occurring compound found in ginger. It serves as a reference standard for the analysis and quantification of 6-gingerol in various samples, such as plant extracts or pharmaceutical preparations. The core function of 6-gingerol is to provide a reliable and consistent reference point for analytical procedures.
Automatically generated - may contain errors
Lab products found in correlation
Glycyrrhizin, by Fujifilm (6 mentions)
Liquiritin, by Fujifilm (4 mentions)
Cinnamaldehyde, by Fujifilm (3 mentions)
Ginsenoside rb1, by Fujifilm (2 mentions)
Capsaicin, by Fujifilm (2 mentions)
Cinnamic acid, by Fujifilm (2 mentions)
Coumarin, by Merck Group (2 mentions)
Berberine, by Fujifilm (1 mentions)
Silica gel 60 f254, by Merck Group (1 mentions)
Trifluoroacetic acid tfa, by Merck Group (1 mentions)
Platycodin d, by ChemFaces (1 mentions)
Quercetin, by Fujifilm (1 mentions)
Eugenol, by Fujifilm (1 mentions)
Naaso2, by Fujifilm (1 mentions)
Sulforaphane, by LKT Laboratories (1 mentions)
Mili q water, by Merck Group (1 mentions)
Diallyl trisulfide, by LKT Laboratories (1 mentions)
Curcumin, by Merck Group (1 mentions)
Naaso2, by Merck Group (1 mentions)
Sulforaphane, by Merck Group (1 mentions)
8 protocols using 6 gingerol
1
Analytical Characterization of Bioactive Compounds
2
Comprehensive Analysis of SBS Constituents
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The reference standards, spinosin (PubChem CID: 155692, purity 98.8%), liquiritigenin (PubChem CID: 114829, purity 99.8%), ginsenoside Rg1 (PubChem CID: 441923, purity 99.3%), and Platycodin D2 (PubChem CID: 53317652, purity 98.0%) were purchased from Biopurify Phytochemicals (Chengdu, China). Liquiritin (PubChem CID: 503737, purity 99.6%), ginsenoside Rb1 (PubChem CID: 9898279, purity 98.0%), glycyrrhizin (PubChem CID: 14982, purity 99.0%), and 6-gingerol (PubChem CID: 442793, purity 98.0%) were purchased from Wako Chemicals (Osaka, Japan). Atractylenolide III (PubChem CID: 155948, purity 99.0%), atractylenolide II (PubChem CID: 14448070, purity 99.0%), and atractylenolide I (PubChem CID: 5321018, purity 99.0%) were purchased from KOC Biotech (Daejeon, Korea). Platycodin D (PubChem CID: 162859, purity 98.0%) and allantoin (PubChem CID: 204, purity 98.0%) were purchased from ChemFaces Biochemical (Wuhan, China) and Merck (Darmstadt, Germany). The chemical structures of these reference standard compounds are shown in Fig. 1 . The HPLC-grade solvents, methanol, acetonitrile, and water, were purchased from J.T. Baker (Phillipsburg, NJ, USA) and trifluoroacetic acid (TFA, for HPLC, ≥99.0%) was purchased from Merck (Darmstadt, Germany).![]()
Chemical structures of the 13 main constituents of SBS.
3
Zebrafish Nrf2 Mutant Model Analysis
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In this study, wild-type (AB strain) and Nrf2-mutant (nfe2l2afh318) [15 (link)] zebrafish larvae were used. The Nrf2-mutant line was maintained by polymerase chain reaction based genotyping, as described previously [28 (link)]. Embryos were obtained by natural mating. Capsaicin, carnosic acid, cinnamaldehyde, eugenol, 6-gingerol, isoeugenol, quercetin, H2O2 and NaAsO2 were purchased from FUJIFILM Wako (Osaka, Japan). Diallyl trisulfide and sulforaphane were bought from LKT Laboratories (St. Paul, MN, USA). Curcumin and 6-MSITC were purchased from Sigma-Aldrich Japan (Tokyo, Japan) and Abcam (Cambridge, UK), respectively. H2O2 and NaAsO2 were dissolved in MiliQ water (Merck-Millipore, Billerica, MA), sulforaphane in ethanol and other phytochemicals were dissolved in dimethyl sulfoxide for the stock solution, and were diluted to the final concentration with E3+ medium (5 mM NaCl, 0.17 mM KCl, 0.33 mM CaCl2, 0.33 mM MgSO4 and 0.1 μg/mL methylene blue).
4
Quantification of Bioactive Compounds
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Cinnamaldehyde (≥98.0%), cinnamic acid (≥98.0%), liquiritin (≥99.0%), 6-gingerol (≥98.0%), and glycyrrhizin (≥99.0%) were purchased from Wako (Osaka, Japan). Amygdalin and coumarin (both ≥99.0%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). liquiritin apioside (≥98.0%) was purchased from Shanghai Sunny Biotech (Shanghai, China) and ephedrine HCl (≥95.0%) was provided from Ministry of Food and Drug Safety. High-performance liquid chromatography (HPLC) grade, methanol, acetonitrile, and water were purchased from J. T. Baker (Phillipsburg, NJ, USA). Reagent grade, trifluoroacetic acid was purchased from Sigma-Aldrich (St. Louis, MO, USA).
5
Quantification of Bioactive Compounds in GJT Extract
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The reference standards, gallic acid, benzoic acid, and coumarin were purchased from Sigma-Aldrich (St. Louis, MO, USA). Albiflorin, paeoniflorin, liquiritin, cinnamic acid, cinnamaldehyde, glycyrrhizin, and 6-gingerol were purchased from Wako (Osaka, Japan). Benzoylpaeoniflorin and liquiritin apioside were purchased from Biopurify Phytochemicals (Chengdu, China) and Shanghai Sunny Biotech (Shanghai, China). A standard stock solution of these components were dissolved in methanol at concentrations of 1.0 mg/mL. For HPLC analysis of the GJT extract, 100 mg of lyophilized GJT extract was dissolved in 20 mL of distilled water and then the solution was diluted to 10-fold for quantitative analysis of paeoniflorin. Solutions were filtered through a SmartPor GHP 0.2 μm syringe filter (Woong Ki Science Co., Seoul, Korea) before application to the column.
6
Quantitative HPLC Analysis of Herbal Compounds
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The following six standard compounds used for quantitative analyses were purchased from the indicated suppliers: liquiritin apioside (Cat No. 74639-14-8, purity: 98.0%) from Shanghai Sunny Biotech (Shanghai, China); glycyrrhizin (Cat No. 1405-86-3, purity: 99.4%), liquiritin (Cat No. 551-15-5, purity: 99.6%), and 6-gingerol (Cat No. 23513-08-8, purity: 98.3%) from Wako Chemicals (Osaka, Japan); and narirutin (Cat No. 14259-46-2, purity: 99.5%) and hesperidin (Cat No. 520-26-3, purity: 98.6%) from Biopurify Phytochemicals (Chengdu, China). The chemical structures of these reference standard compounds, used for the quantitative high-performance liquid chromatography (HPLC) analysis of YJT samples, are shown in Supplementary Figure 1 . Solvents (methanol, acetonitrile, and water) used for simultaneous analyses were HPLC grade and were purchased from JT Baker (Phillipsburg, NJ, USA). Formic acid (ACS reagent grade, Cat No. 64-18-6, and purity: 100.0%) and dimethyl sulfoxide (ACS reagent grade, Cat No. 67-68-5, and purity: 99.9%) were purchased from Merck KGaA (Darmstadt, Germany).
7
Quantification of Bioactive Compounds in Herbal Medicines
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Ginsenoside Rg1, ginsenoside Rb1, glycyrrhizin, and 6-gingerol were purchased from Wako (Osaka, Japan). Liquiritin was obtained from NPC BioTechnology Inc. (Daejeon, Korea). The purities of all reference compounds were ≥98.0% according to HPLC analysis. HPLC-grade methanol, acetonitrile, and water were obtained from J.T. Baker (Phillipsburg, NJ, USA). Glacial acetic acid was of analytical reagent grade and procured from Junsei (Tokyo, Japan). The crude herbal medicines from Ginseng Radix Alba, Zingiberis Rhizoma, Glycyrrhizae Radix et Rhizoma, and Atractylodis Rhizoma Alba were purchased from Omniherb (Yeongcheon, Korea) and HMAX (Jecheon, Korea). The origin of each herbal medicine was taxonomically confirmed by Prof. Je Hyun Lee, Dongguk University, Gyeongju, Korea. Voucher specimens (2008-KE19-1 through KE19-4) have been deposited at the Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine.
8
Natural Compounds for Pain Research
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Capsaicin, [6]-shogaol and [6]-gingerol were obtained from Wako chemicals (Tokyo, Japan). RTX was obtained from LC laboratories (Woburn, MA).
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