Bgc803

The GC cell lines SGC7901, BGC803, AGS, MKN45, BGC823, MGC803, HGC27 and the gastric mucosal epithelial cell lines GES-1 were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). SGC7901, AGS, BGC823, MKN45, BGC803, MGC803, HGC27, and GES-1 cells were cultured in RPMI-1640 medium (Gibco, Grand Island, NY, USA). All media were supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin (Sigma-Aldrich, St Louis, MO, USA), and 100 mg/mL streptomycin (Sigma-Aldrich). Cycloheximide (CHX), proteasome inhibitor MG132, chloroquine diphosphate (CQ), and GSK-3β inhibitor CHIR-99021 were bought from Selleck Chemicals (Houston, TX, USA). Alkaline phosphatase (CIP) was obtained from Roche (Roche Applied Science, Indianapolis, IN, USA). The GSK-3β inhibitor LiCl was obtained from Sigma Aldrich.

Six gastric adenocarcinoma cell lines (AGS, BGC-823, BGC-803, SGC-7901, HGC-27, MKN-1) and the normal human epithelial cell line (GES-1) were purchased from the Institute of Biochemistry and Cell Biology of the Chinese Academy of Sciences (Shanghai, China). AGS, BGC-823, BGC-803, HGC-27, MKN-1 cells were cultured in RPMI 1640; SGC-7901 and GES-1 cells were cultured in DMEM (GIBCO-BRL) medium supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin and 100 mg/ml streptomycin (Invitrogen, Carlsbad, CA, USA), and maintained at 37 °C with 5% CO₂.

For cell lines, human GC cell lines, BGC803, MKN45, SGC7901, and MKN28, were cultured in RPMI-1640 medium (GIBCO BRL, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (GIBCO BRL) and 100 mg/ml penicillin-streptomycin. Human GC cell line AGS was cultured in DMEM (GIBCO BRL) supplemented with 10% fetal bovine serum (GIBCO BRL) and 100 mg/ml penicillin-streptomycin. HEK293T cells were cultured in DMEM (GIBCO BRL) supplemented with 10% fetal bovine serum (GIBCO BRL) and 100 mg/ml penicillin-streptomycin. Cells were incubated at 37 °C in 5% CO2. Human GC cell lines BGC803, MKN45, SGC7901, MKN28, and AGS, were purchased from Genechem (Shanghai, China). HEK293T cells were purchased from ATCC (Manassas, VA, USA). For drugs, OSI906 and Doxycycline were purchased from Sellechem catalog #S1091 and #S4163, respectively, and the IC50 data was obtained from the Selleckchem website (Selleckchem.com). Heclin was purchased from Sigma-Aldrich (SML1396).