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Glycerol colorimetric assay kit

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The Glycerol Colorimetric Assay Kit is a laboratory tool designed to quantitatively measure the concentration of glycerol in various samples. The kit utilizes a colorimetric method to determine the glycerol level, providing a reliable and efficient means of analysis.

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Spelling variants of this product

Colorimetric assay kit (177 citations) Colorimetric assay (69 citations) Glycerol (9 citations) Glycerol Assay Kit (5 citations) Glycerol kit (2 citations)

27 protocols using glycerol colorimetric assay kit

1

Quantifying Serum Lipid Levels

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Serum levels of free fatty acids, glycerol, total cholesterol, and triglycerides were measured using free fatty acid quantification kits (ab65341, Abcam, Cambridge, UK), a glycerol colorimetric assay kit (10010755, Cayman Chemical, Ann Arbor, MI, USA), a total cholesterol colorimetric assay kit (MBS2540484, Mybiosource, San Diego, USA), and a triglyceride colorimetric assay kit (10010303, Cayman Chemical), respectively.
Choi E.W., Kim H.J., Jung Y.C., Go H.S, & Seong J.K. (2022). Effects of high fat diet-induced obesity on pathophysiology, immune cells, and therapeutic efficacy in systemic lupus erythematosus. Scientific Reports, 12, 18532.
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2

Adipose Tissue Free Fatty Acid Assay

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0.3 g of adipose tissue was minced and incubated with 500 μl assay buffer at 37°C for 1 hour. The tissue mixture was centrifuged at 500 × g for 10 minutes and the liquid between the pellet and floating adipose tissue was moved to a new tube. Free fatty acids were measured using kits obtained from Wako Diagnostics (Mountain View, CA) and glycerol was measured using a Glycerol Colorimetric Assay Kit from Cayman Chemical Company (Ann Arbor, MI), each sample was measured in triplicates and presented as a composite mean with standard error of the mean.
Jahansouz C., Xu H., Hertzel A.V., Kizy S.S., Steen K.A., Foncea R., Serrot F.J., Kvalheim N., Luthra G., Ewing K., Leslie D.B., Ikramuddin S, & Bernlohr D.A. (2017). Partitioning of Adipose Lipid Metabolism by Altered Expression and Function of PPAR Isoforms After Bariatric Surgery. International journal of obesity (2005), 42(2), 139-146.
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3

Phosphate and Glycerol Quantification in SCCs

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The total phosphate content of SCCs was determined by the malachite green method following digestion with perchloric acid, as previously described21 (link). To determine glycerol, SCCs were incubated with 2 N HCl at 100°C for 2 hours. The samples were neutralized with NaOH in the presence of 62.5 mM HEPES pH 7.5. Glycerol concentration was measured using the Glycerol Colorimetric assay kit (Cayman Chemical) according to the manufacturer’s protocol.
Zamakhaeva S., Chaton C.T., Rush J.S., Castro S.A., Kenner C.W., Yarawsky A.E., Herr A.B., van Sorge N.M., Dorfmueller H.C., Frolenkov G.I., Korotkov K.V, & Korotkova N. (2021). Modification of cell wall polysaccharide guides cell division in Streptococcus mutans. Nature chemical biology, 17(8), 878-887.
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4

Glycerol Colorimetric Assay Protocol

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Samples for glycerol measurement were prepared as described for the phosphate assay but were not digested with alkaline phosphatase. Instead glycerol concentration was measured using the Glycerol Colorimetric assay kit (Cayman Chemical) according to the manufacturer’s protocol.
Edgar R.J., van Hensbergen V.P., Ruda A., Turner A.G., Deng P., Breton Y.L., El-Sayed N.M., Belew A.T., McIver K.S., McEwan A.G., Morris A.J., Lambeau G., Walker M.J., Rush J.S., Korotkov K.V., Widmalm G., van Sorge N.M, & Korotkova N. (2019). Discovery of glycerol phosphate modification on streptococcal rhamnose polysaccharides. Nature chemical biology, 15(5), 463-471.
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5

Glycerol Release from Gonadal Fat

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Gonadal fat pads were excised from mice, cut into 20 mg pieces, and incubated at 37°C in Krebs–Ringer solution pH 7.4, containing 12 mM HEPES, 4.9 mM KCl, 121 mM NaCl, 1.2 mM MgSO4, 0.33 mM CaCl2, 3.5% (w/v) fatty acid‐free BSA, and 0.1% (w/v) glucose. No stimulation was performed. Released glycerol was measured from supernatants after 4 h incubation using the Glycerol Colorimetric Assay Kit (Cayman), and tissue weights were used for normalization.
Erdem M., Möckel D., Jumpertz S., John C., Fragoulis A., Rudolph I., Wulfmeier J., Springer J., Horn H., Koch M., Lurje G., Lammers T., Olde Damink S., van der Kroft G., Gremse F, & Cramer T. (2019). Macrophages protect against loss of adipose tissue during cancer cachexia. Journal of Cachexia, Sarcopenia and Muscle, 10(5), 1128-1142.
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6

Measuring Muscle and Plasma Cytokines

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Plasma and gastrocnemius IL-6 concentrations were measured using a R&D Mouse IL-6 ELISA Duo set (R&D Systems, Minneapolis, MI, USA) according to the manufacturer’s instructions. Gastrocnemius IL-6 concentration was related to total protein concentration measured using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, Rockford, IL, USA) according to the manufacturer’s instructions. Plasma glycerol was measured using the Glycerol Colorimetric Assay Kit (Cayman Chemical Co., Ann Arbor, MI, USA).
Ma S., Huang Q., Tominaga T., Liu C, & Suzuki K. (2018). An 8-Week Ketogenic Diet Alternated Interleukin-6, Ketolytic and Lipolytic Gene Expression, and Enhanced Exercise Capacity in Mice. Nutrients, 10(11), 1696.
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7

Plasma Lipid and Glucose Analysis

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To determine the lipid profile and glucose level in the plasma, standard tests from Biomaxima S.A. (Poland) were used. To determine the glycerol level in the plasma, a Glycerol Colorimetric Assay Kit (Cayman, USA) was used.
Dudek M., Knutelska J., Bednarski M., Nowiński L., Zygmunt M., Mordyl B., Głuch-Lutwin M., Kazek G., Sapa J, & Pytka K. (2015). A Comparison of the Anorectic Effect and Safety of the Alpha2-Adrenoceptor Ligands Guanfacine and Yohimbine in Rats with Diet-Induced Obesity. PLoS ONE, 10(10), e0141327.
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8

Adipose Tissue Free Fatty Acid Assay

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0.3 g of adipose tissue was minced and incubated with 500 μl assay buffer at 37°C for 1 hour. The tissue mixture was centrifuged at 500 × g for 10 minutes and the liquid between the pellet and floating adipose tissue was moved to a new tube. Free fatty acids were measured using kits obtained from Wako Diagnostics (Mountain View, CA) and glycerol was measured using a Glycerol Colorimetric Assay Kit from Cayman Chemical Company (Ann Arbor, MI), each sample was measured in triplicates and presented as a composite mean with standard error of the mean.
Jahansouz C., Xu H., Hertzel A.V., Kizy S.S., Steen K.A., Foncea R., Serrot F.J., Kvalheim N., Luthra G., Ewing K., Leslie D.B., Ikramuddin S, & Bernlohr D.A. (2017). Partitioning of Adipose Lipid Metabolism by Altered Expression and Function of PPAR Isoforms After Bariatric Surgery. International journal of obesity (2005), 42(2), 139-146.
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9

Adipocyte RNA Extraction and Gene Expression Analysis

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Qiagen RNeasy mini kit was used for extraction of RNA from adipocytes. cDNA was synthesized using iScript™ (Biorad). Gene expression assays (ABI Life Technology) were used in PCR. PrimePCR DNA contamination assay from Biorad was used to determine genomic contamination of cDNA. Human subcutaneous adipocytes were purchased from Zenbio Inc. 199/EBSS Hyclone medium (Thermo Scientific) was used in incubation of human adipose tissue explants. NEFA-HR (2) kit from Wako and Glycerol colorimetric assay kit from CaymanChemicals was employed when measuring lipolysis.
Møller C.L., Pedersen S.B., Richelsen B., Conde-Frieboes K.W., Raun K., Grove K.L, & Wulff B.S. (2015). Melanocortin agonists stimulate lipolysis in human adipose tissue explants but not in adipocytes. BMC Research Notes, 8, 559.
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10

Caco-2 Permeability of Glycerol and Inhibitors

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Caco-2 cells were plated in 24-well Transwell plates and incubated for 7, 14, and 21 days. After being removed from the culture medium, the cells were treated with HBSS (pH 6.0) on the apical side and HBSS (pH 7.4) on the basal side and preincubated at 37 °C in an atmosphere of 5% CO2 for 15 min. The cells were then treated on the apical side with glycerol (final concentration: 50 mM), a combination of glycerol and HgCl2 (final concentration: 10 µM), or a combination of glycerol and phloretin (final concentration: 100 µM). Samples were collected from the basal side up to 90 min after treatment, and the concentration of glycerol was determined using a glycerol colorimetric assay kit (Cayman Chemical, Ann Arbor, MI, USA) [52 (link),53 (link)]. The Papp was then calculated based on the amount of glycerol that had permeated to the basal side [54 (link)].
Ikarashi N., Nagoya C., Kon R., Kitaoka S., Kajiwara S., Saito M., Kawabata A., Ochiai W, & Sugiyama K. (2019). Changes in the Expression of Aquaporin-3 in the Gastrointestinal Tract Affect Drug Absorption. International Journal of Molecular Sciences, 20(7), 1559.
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