Protein extraction solution
Protein extraction solution is a reagent designed to isolate and extract proteins from biological samples. It functions by disrupting cellular membranes and releasing proteins into the solution, enabling their subsequent analysis or purification.
Lab products found in correlation
2 protocols using protein extraction solution
Protein Expression Analysis in Eye
Western Blot Analysis of Metallomacrocycle 7
60 mm dish) were plated in dishes and allowed to attach for
24 h. After metallomacrocycle
washed with cold PBS, harvested, and cell pellets were lysed with
the protein extraction solution (iNtRON, Korea) on ice for 1 h. Proteins
were separated by centrifugation at 12 000 rpm for 10 min at
4 °C, and aliquots (40 μg) were separated by 12% sodium
dodecyl sulfate-polyacrylamide gel (SDS-PAGE) electrophoresis and
then transferred onto nitrocellulose membranes (Bio-Rad Laboratories
Inc., Hercules, CA). Nonspecific binding was blocked by incubating
membranes with 5% skim milk in TBST (10 nmol/L tris–HCl (pH
8.0), 150 mmol/L NaCl, and 0.1% Tween 20) for 1 h at room temperature
(rt). Membranes were then incubated with 1:1000 diluted primary antibodies
at 4 °C overnight, probed with horseradish peroxidase-conjugated
secondary antibodies (1:5000), and visualized by densitometry (ECL
system, Amersham, U.K.) according to the manufacturer’s instructions.
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