Anti ly6c pe cy7

Manufactured by Thermo Fisher Scientific

The Anti-Ly6C PE-Cy7 is a flow cytometry reagent that binds to the Ly6C antigen expressed on certain immune cell populations. It is a conjugate of the anti-Ly6C antibody with the PE-Cy7 fluorescent dye, enabling the detection and analysis of Ly6C-positive cells using flow cytometry.

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Lab products found in correlation

Anti cd45 fitc, by Thermo Fisher Scientific (1 mentions) Fc block reagent, by BD (1 mentions) Anti cd62l percp cy5, by BD (1 mentions) Anti f4 80 efluor450, by Thermo Fisher Scientific (1 mentions) Anti ccr2 apc, by R&D Systems (1 mentions) Anti ly6g buv395, by BD (1 mentions) Anti f4 80 bv421, by BioLegend (1 mentions) Anti cd11b buv395, by BD (1 mentions) Facscanto instrument, by BD (1 mentions) Efluor 780, by Thermo Fisher Scientific (1 mentions) Ab219800, by Abcam (1 mentions) Ab76774, by Abcam (1 mentions) Anti cd11b fitc, by Thermo Fisher Scientific (1 mentions) Ab209845, by Abcam (1 mentions) Alcian blue staining kit, by Solarbio (1 mentions) Anti asc sc 22514, by Santa Cruz Biotechnology (1 mentions) Pas staining kit, by Wuhan Servicebio Technology (1 mentions) Anti cd11b pe, by BD (1 mentions) Anti cd45 fluorescein isothiocyanate fitc, by Thermo Fisher Scientific (1 mentions) Anti cd45 af700, by Thermo Fisher Scientific (1 mentions)

2 protocols using anti ly6c pe cy7

Flow Cytometric Phenotyping of Macrophages

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Monocyte-derived macrophages or BMDMs were harvested and washed in cold PBS. Cells were incubated in Fc-block reagent (BD biosciences) and fixable viability dye eFluor 780 (Invitrogen) for 15 min at 4°C in cold PBS. Monocyte-derived macrophages were washed once and stained with the following antibodies: anti-CD62L PerCp-Cy5.5 (BD biosciences), anti-CCR2 APC (R and D Systems), anti-F4/80 efluor450 (eBioscience), anti-CSF1R BV711 (Biolegend), anti-Ly6G BUV395 (BD biosciences), anti-CD11b BUV737 (BD biosciences), anti-MHCII(I-A/I-E) PE (eBioscience) and anti-Ly6C PE-Cy7 (eBioscience). BMDMs were washed once and stained with the following antibodies: anti-CD45 FITC (eBioscience), anti-F4/80 BV421 (Biolegend), anti-CD11b BUV395 (BD biosciences). Stained cells were analyzed on by flow cytometry using a BD FACS CANTO instrument. Loss of eGFP or CD45 was assessed by gating on live F4/80 + macrophages using FlowJo X.

Lim J., Park H., Heisler J., Maculins T., Roose-Girma M., Xu M., Mckenzie B., van Lookeren Campagne M., Newton K, & Murthy A. (2019). Autophagy regulates inflammatory programmed cell death via turnover of RHIM-domain proteins. eLife, 8, e44452.

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Antibody Staining Protocol for Immune Cell Analysis

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Antibodies to GSDMD (ab219800 and ab209845) and MUC2 (AB76774) were from Abcam. Anti–α-tubulin (T9026) and anti–β-actin (A1978) were from Sigma-Aldrich. Anti–caspase-11 (NB120-10454) was supplied from Novus Biologicals. Anti-ASC (sc-22514) was from Santa Cruz Biotechnology. Anti-phosphorylated STING (72971s), anti-STING (13647s), anti-phosphorylated IRF3 (4947s), anti-IRF3 (4302s), anti-TBK1 (3013s), and anti-phosphorylated TBK1 (5483s) were from Cell Signaling Technology. DSS (DB001-38) was from TdB Consultancy. PAS staining kit was from Servicebio (G1008). Alcian blue staining kit was from Solarbio (G1560). Anti–CD45–fluorescein isothiocyanate (FITC) (30-F11,11-0451-82), anti–CD8a–phycoerythrin (PE) (53-6.7,12-0081-83), anti–CD45-AF700 (30-F11,85-11-0112-81), anti–CD11b-FITC (M1/70,85-12-0114-81), anti-F4/80 (BM8,17-4801-82), anti–Ly6c-PE-Cy7 (HK1.4,25-5932-82), anti–Ly6g–eFlour 450 (1A8-LY6G,48-9668-82), and FVD (fixable viability dyes)–eFlour 506 (65-0866) were from eBioscience. Anti–CD4-APC (allophycocyanin)–Cy7 (GK1.5,100414) was from BioLegend. Anti–CD11b-PE (M1/70, 557397) was from BD Biosciences.

Ma C., Yang D., Wang B., Wu C., Wu Y., Li S., Liu X., Lassen K., Dai L, & Yang S. (2020). Gasdermin D in macrophages restrains colitis by controlling cGAS-mediated inflammation. Science Advances, 6(21), eaaz6717.

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