Pe cy7 labeled anti cd62l

5 × 10⁵human T cells were infected by the lentivirus mentioned above. To draw cell growth curves, live T cells were counted using trypan blue staining and Automated Cell Counter (Thermo Fisher, Countess™ 3). Expression of CD19-, BCMA-, BC19- or 19BC CAR was evaluated by Biotinylated protein L (Acro Biosystems, Cat: RPL-P814R, Lot: BL11R-76EF1-GY,1:400 dilution) and APC--conjugated Streptavidin (Biolegend, Cat: 405207, Lot: B388667, 1:100 dilution). PE-labeled anti-CD45RA (Biolegend, Clone: HI100, Cat: 304107, Lot: B378521, 1:100 dilution) and PE-Cy7-labeled anti-CD62L (Biolegend, Clone: DREG-56, Cat: 304821, Lot: B373156, 1:100 dilution) were employed to determine phenotypes of infusion products of CAR T, including naïve (CD45RA⁺ CD62L⁺), central memory (CD45RA⁻ CD62L⁺), effector memory (CD45RA⁻ CD62L⁻), and effector (CD45RA⁺ CD62L⁻) T cells.

Biotinylated protein L (Acro Biosystems, Cat: RPL-P814R, Lot: BL11R-76EF1-GY,1:400 dilution) and APC--conjugated Streptavidin (Biolegend, Cat: 405207, Lot: B388667, 1:100 dilution). PE-labeled anti-CD45RA (Biolegend, Clone: HI100, Cat: 304107, Lot: B378521, 1:100 dilution) and PE-Cy7-labeled anti-CD62L (Biolegend, Clone: DREG-56, Cat: 304821, Lot: B373156, 1:100 dilution); CellTracker Deep Red (Thermo Fisher Scientific, Cat: C34565, Lot: 1987253, CTDR, 1:40,000 dilution).