Anti baffr apc

Manufactured by BioLegend

Sourced in United States

Anti-BAFFR-APC is a flow cytometry reagent that binds to the B cell-activating factor receptor (BAFFR) on the surface of cells. BAFFR is a key receptor involved in the survival and maturation of B cells. The Anti-BAFFR-APC conjugate allows for the detection and analysis of BAFFR-expressing cells by flow cytometry.

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Lab products found in correlation

Anti cd20 fitc, by BioLegend (1 mentions) Anti traf2, by Santa Cruz Biotechnology (1 mentions) Anti bcma pe, by BioLegend (1 mentions) Anti taci pe, by BioLegend (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Zhejiang Tianhang Biotechnology (1 mentions) Anti β actin, by ZSGB-BIO (1 mentions) Rituximab, by Roche (1 mentions) Anti cd19 fitc, by BioLegend (1 mentions) Anti baff apc, by BioLegend (1 mentions)

2 protocols using anti baffr apc

Evaluation of BAFF Signaling Pathway in B-cell

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CP-25 was supplied by the Chemistry Laboratory of Institute of Clinical Pharmacology of Anhui Medical University (Hefei, Anhui Province, China). Rituximab (Roche Pharma, Switzerland); Etanercept (Shanghai Guojian Pharmaceutical, Co., Ltd., China); BAFF (Pepro Tech, United States); anti-CD19-APC/PE/FITC, anti-CD20-FITC, anti-CD27-PE/APC, anti-BAFFR-APC, anti-BCMA-PE, and anti-TACI-PE (Biolegend, United States); anti-β-actin (ZSGB-BIO, China); Anti-TRAF2 (Santa Cruz, China); Anti-Phospho-NF-κBp65, anti-p100/52, and anti-Phospho-p38 MAPK (Cell Signaling Technology, United States); RPMI 1640 medium (Gibco, United States); fetal bovine serum (Zhejiang Tianhang Biotechnology, China).

Zhang F., Shu J.L., Li Y., Wu Y.J., Zhang X.Z., Han L., Tang X.Y., Wang C., Wang Q.T., Chen J.Y., Chang Y., Wu H.X., Zhang L.L, & Wei W. (2017). CP-25, a Novel Anti-inflammatory and Immunomodulatory Drug, Inhibits the Functions of Activated Human B Cells through Regulating BAFF and TNF-alpha Signaling and Comparative Efficacy with Biological Agents. Frontiers in Pharmacology, 8, 933.

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Immunophenotyping of BAFF and BAFFR

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Cells were harvested as previously described and stained for 30 min at 4 °C with anti-BAFF-APC (#366,507; BioLegend), anti-BAFFR-APC (#316,916; BioLegend), and anti-CD19-FITC antibodies (#392,508; BioLegend). Cells were acquired and analyzed using flow cytometry (BD Biosciences).

Li G., Zhang Q., Liu Z., Shen H., Zhu Y., Zhou Z., Ding W., Han S., Zhou J., Ou R., Luo M, & Liu S. (2021). TriBAFF-CAR-T cells eliminate B-cell malignancies with BAFFR-expression and CD19 antigen loss. Cancer Cell International, 21, 223.

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