NSCLC tissues or cells were lysed by ice-cold lysis buffer (Cell Signaling Technology, Danvers, MA, USA). After quantitation, the extracted protein was loaded onto 10% SDS-polyacrylamide gel electrophoresis and then electroblotted onto clear blot membranes (ATTO, Tokyo, Japan). After blocking by 5% skim milk, the transferred membranes were probed with antibodies at 4°C for 4 h, including anti-TNFAIP8 (ab195810; 1 : 1500 dilution; Abcam, Cambridge, MA, USA), anti-Proliferating Cell Nuclear Antigen (PCNA; ab92552; 1 : 1500 dilution; Abcam), anti-matrix metalloproteinases 13 (MMP 13; ab51072; 1 : 1500 dilution; Abcam), anti-Cleaved Caspase-3 (c-caspase 3; ab32351; 1 : 1500 dilution; Abcam), and anti-β-actin (ab8227; 1 : 2000 dilution; Abcam) was served as a loading reference. After incubating with horseradish peroxidase-conjugated secondary antibodies (ab1500771; 1 : 3000 dilution; Abcam), the blots were visualized by enhanced chemiluminescence detection kit (GE Healthcare, Piscataway, NJ, USA).
Ab195810
Manufactured by Abcam
Sourced in United States
Ab195810 is a laboratory equipment product. It serves a core function, but further details on its intended use are not available.
Automatically generated - may contain errors
Lab products found in correlation
Ab32351, by Abcam (1 mentions)
Ab1500771, by Abcam (1 mentions)
Ab92552, by Abcam (1 mentions)
Ab51072, by Abcam (1 mentions)
Enhanced chemiluminescence detection kit, by GE Healthcare (1 mentions)
Lysis buffer, by Cell Signaling Technology (1 mentions)
Ab8227, by Abcam (1 mentions)
Ab104836, by Abcam (1 mentions)
Ab219372, by Abcam (1 mentions)
Ab8226, by Abcam (1 mentions)
Ab205718, by Abcam (1 mentions)
Ab16667, by Abcam (1 mentions)
Ab32389, by Abcam (1 mentions)
Ecl system, by Beyotime (1 mentions)
Bicinchoninic acid kit, by Beyotime (1 mentions)
2 protocols using ab195810
1
Protein Expression Analysis of NSCLC
2
Quantifying Protein Expression in Thyroid Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Thyroid cancer cells were lysed and bicinchoninicacid kit (Beyotime, Shanghai, China) was used for protein concentration determination. The proteins were quantified, and then a total of 40 μg protein samples were separated by SDS-PAGE) gel. The proteins were transferred onto a polyvinylidene fluoride (PVDF) membrane. The PVDF membrane was blocked by 5% skim milk for 1 h following by incubation with the following antibodies, including Ki67 (ab16667, Abcam), P53 (ab32389, Abcam), matrix metallopeptidase 9 (MMP9; ab219372, Abcam), hexokinase 2 (HK-2; ab104836, Abcam), TNFAIP8 (ab195810, Abcam) and β-actin (ab8226, Abcam). The PVDF membrane was probed with the secondary antibody (ab205718, Abcam). The protein signal was measured and quantified via the enhanced chemiluminescent (ECL) system (Beyotime) and Image J software.
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