Sst microtainer tubes

Manufactured by BD

Sourced in United States

The SST Microtainer tubes are blood collection devices designed for the collection, separation, and storage of serum samples. They feature a gel separator that allows for the effective separation of serum from the cellular components of the blood sample during centrifugation.

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Lab products found in correlation

Balb c mice, by Janvier Labs (2 mentions) Tetramethylbenzidine tmb, by Thermo Fisher Scientific (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Ab65390, by Abcam (1 mentions) Sta 396, by Cell Biolabs (1 mentions) Spectramax m2, by Molecular Devices (1 mentions) Au480, by Beckman Coulter (1 mentions) Logiq e9, by GE Healthcare (1 mentions) Brilliance 64, by Philips (1 mentions)

Spelling variants of this product

BD Microtainer (240 citations) Microtainer tubes (239 citations) BD Microtainer SST tubes (55 citations) SST tubes (48 citations) BD Microtainer SST (13 citations) BD Microtainer SST blood collection tubes (3 citations) BD Microtainer SST amber blood collection tubes (3 citations) BD microtainer SSTTM tubes (2 citations)

5 protocols using sst microtainer tubes

Immunogenicity Assessment of Vaccine Formulations

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Immunogenicity of the described vaccine formulations was studied in 8 week old female BALB/c mice (Janvier). Groups of five mice were immunized three times by the subcutaneous (s.c.) route in the scruff of the neck with 100 μl of the adjuvanted proteins in three week intervals. Prior to the first immunization as well as before every new immunization mice were bled by the tail vein and serum gained by centrifugation of the blood in SST Microtainer tubes (Becton, Dickinson and Company). An additional blood collection was performed three weeks and six months after the last immunization.

Bolz M., Bénard A., Dreyer A.M., Kerber S., Vettiger A., Oehlmann W., Singh M., Duthie M.S, & Pluschke G. (2016). Vaccination with the Surface Proteins MUL_2232 and MUL_3720 of Mycobacterium ulcerans Induces Antibodies but Fails to Provide Protection against Buruli Ulcer. PLoS Neglected Tropical Diseases, 10(2), e0004431.

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Serum-based Virus-specific ELISA and HI Assay

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Blood was collected in SST Microtainer tubes (Becton, Dickinson & Company) using mandibular bleeds. Serum was isolated by centrifugation and frozen at −40°C until testing. Serum was treated with Receptor Destroying Enzyme (Denka Seiken Company) at 1:4 ratio and incubated for 18 h at 37°C and at 56°C for 30 min.
Virus-specific ELISA was performed by coating flat-bottom immuno 96-well plates (Fisher) overnight with 50 hemagglutinin units (HAU) of homologous virus in 50 μL per well. After blocking with 4% BSA (Sigma-Aldrich) in 1×PBS/0.05% Tween (PBST) (Gibco, Life Technologies), serum was serial diluted twofold or fourfold from 1:100 dilution and secondary HRP-conjugated anti-IgM or IgG (Southern Biotech) was added, respectively. After PBST wash, 3,3′,5,5′-Tetramethylbenzidine (TMB) (eBioscience) and Stop solution (Kirkegaard & Perry) (50 μL) were added at a 1:1 ratio. ELISA background was subtracted, and endpoint titers with optical density (OD) values reaching ≥0.1 were plotted. The limit of detection for ELISA titers was 3.32 log₄ units for IgG and 6.64 log₂ units for IgM. Hemagglutination inhibition (HI) assay with 1% turkey red blood cells was performed as previously described (27 (link)). The limit of detection for HI titers was 2.32 log₂ HI units/mL.

Liepkalns J.S., Pandey A., Hofstetter A.R., Kumar A., Jones E.N., Cao W., Liu F., Levine M.Z., Sambhara S, & Gangappa S. (2016). Rapamycin Does Not Impede Survival or Induction of Antibody Responses to Primary and Heterosubtypic Influenza Infections in Mice. Viral immunology, 29(8), 487-493.

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Evaluation of VRP Vaccine Protocols

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All animal studies were conducted in 8 weeks old female BALB/c mice (Janvier). VRPs (10⁷ fluorescence-forming units) were either applied subcutanously (s.c.) in the neck (for evaluation of the vaccination protocol) or intramuscularly (i.m.) into the right caudal tight muscle using a volume of 100 μl or 30 μl, respectively. When several immunizations were conducted, injections were performed in three week intervals. For prime-boost vaccination regimen, 30 μg of non-adjuvanted recombinant protein were applied s.c. into the neck. Blood was collected from the tail vein prior to every immunization as well as 5/6 and 14 days after the protein boost. Serum was prepared by centrifugation of the blood in SST Microtainer tubes (Becton, Dickinson and Company).

Bolz M., Kerber S., Zimmer G, & Pluschke G. (2015). Use of Recombinant Virus Replicon Particles for Vaccination against Mycobacterium ulcerans Disease. PLoS Neglected Tropical Diseases, 9(8), e0004011.

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Hepatic Lipid Profiling and Serum Analysis

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The blood collected from the abdominal vena cava was divided into 0.6 mL SST microtainer tubes (BD, Franklin Lakes, NJ, USA), and the blood contained in the SST tubes was completely solidified. Each tube was centrifuged at 4 °C at 12,000 rpm for 2 min, and serum was collected. The liver was immediately harvested, rinsed with physiological saline solution, weighted, and stored at −70 °C until analysis. The triglyceride (TG), total cholesterol (TC), HDL—cholesterol (HDL—C), LDL—cholesterol (LDL—C), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and glucose of the serum were analyzed by blood chemistry analyzer (AU480, Beckman Coulter, Germany). Hepatic TG (STA396, Cell Biolabs, San Diego, CA, USA) and TC (ab65390, Abcam, UK) were evaluated by commercial kits. The absorbance was measured at 450 nm (SpectraMax M2, Molecular Devices, San Jose, CA, USA).

Lee J.Y., An M., Heo H., Park J.Y., Lee J, & Kang C.H. (2023). Limosilactobacillus fermentum MG4294 and Lactiplantibacillus plantarum MG5289 Ameliorates Nonalcoholic Fatty Liver Disease in High-Fat Diet-Induced Mice. Nutrients, 15(8), 2005.

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Preoperative and Postoperative Brain Imaging in Neonatal Congenital Heart Disease

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Routine clinical care was not affected or altered by this study. As is customary with neonates with CHD scheduled for cardiac surgery at Children’s National Medical Center, the majority of study patients underwent preoperative brain imaging in order to establish a baseline. Postoperative brain imaging was obtained based on clinical need. Radiologic imaging was achieved with MRI (General Electric Discovery MR750 3.0T; General Electric), ultrasound (General Electric Logiq E9; General Electric), or CT scan (Philips Brilliance 64; Philips, Andover, MA). Images were read by neuroradiologists blinded to the study and results were not reviewed by the investigators until completion of the study.
Following enrollment, 0.5 mL of blood was collected via an indwelling arterial catheter or central venous catheter prior to surgery. Subsequent blood samples (0.5 mL) were collected 6, 24, 48, 72, and 96 hours after surgery. All samples were immediately placed into collection tubes (SST Microtainer Tubes, BD and Company, Franklin Lakes, NJ) and centrifuged at 6,000 rpm for 2 minutes. Serum was removed from the collection tubes and stored at −80°C until analysis.

Jain P., Spaeder M.C., Donofrio M.T., Sinha P., Jonas R.A, & Levy R.J. (2014). Detection of Alpha II-Spectrin Breakdown Products in the Serum of Neonates With Congenital Heart Disease. Pediatric critical care medicine : a journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies, 15(3), 229-235.

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