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26xlexaop2 mcd8 gfp attp2

The 26XLexAop2-mCD8::GFP}attP2 is a genetic construct that allows for the expression of the mCD8::GFP fusion protein under the control of the LexAop2 promoter. This construct can be used to visualize and study the expression patterns of genes or cell types in Drosophila models.

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2 protocols using 26xlexaop2 mcd8 gfp attp2

1

Standardized Drosophila Rearing Protocol

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A standard cornmeal-molasses diet was used to maintain all fly strains (http://flystocks.bio.indiana.edu/Fly_Work/media-recipes/molassesfood.htm). The following strains were used: w[*]; ry[506] Sb[1] P{ry[+t7.2]=Δ2-3}99B/TM2, Ubx (Bloomington 1798), w[*]; P{y[+t7.7] w[+mC]=26XLexAop2-mCD8::GFP}attP2 (Bloomington 32207), y[1],w[1118]; P{w[mC]=LHG]StanEx[1]} (Bloomington 66673), w[*]; L[*]/CyO; ftz[*] e[*]/TM6,Tb[*].
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2

Drosophila Genetic Strains for Nephrocyte Studies

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All fly strains were maintained on a standard cornmeal-molasses diet (http://flystocks.bio.indiana.edu/Fly_Work/media-recipes/molassesfood.htm). The following strains were used: y[1],w[1118] (Bloomington 6598), w[*]; ry[506] Sb[1] P{ry[+t7.2]= Δ2-3}99B/TM6B, Tb[1] (Bloomington 1798), w[*]; P{y[+t7.7] w[+mC]=26XLexAop2-mCD8::GFP}attP2 (Bloomington 32207), w[*]; L[*]/CyO; ftz[*] e[*]/TM6B,Tb[*],Antp[Hu]; StanEx1 is the X-linked index insertion of the StanEx enhancer trap P-element collection utilizing the P-element promoter–LexA DNA binding domain “L”–Gal4 hinge region “H”–Gal4 transcriptional activation domain “G” construct (see above), y[1],w[1118], P{w[mC]=LHG]StanEx[1]}. We noted LexA-independent detection of 26xLexAop2-CD8::GFP in garland and pericardial nephrocytes at the L3 stage (Supplemental Material, Figure S6).
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