Acc c83b10 3676s

GN (≥98% purity) was obtained from ChemFaces (Wuhan, China). Antibodies against SREBP-1c (H-160; sc-8984), PPARα (H-2; sc-398394), PGC-1α (H-300; sc-13067), IκBα (H-4; sc-164), NF-κB p65 (F-6; sc-8008p65), SIRT1 (H-300; sc-15404), AMPK α1/2 (D-6; sc-74461), and β-actin (C4; sc-47778) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Specific primary antibodies against p-ACC (Ser79; #3661s) and ACC (C83B10; #3676s) were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against CYP2E1 (ab28146) and p-AMPK α1 (Thr172; PA5-17831) were purchased from Abcam (Cambridge, MA, USA) and Invitrogen (Rockford, IL, USA), respectively. Ethanol and Ex52735 were obtained from Sigma (St. Louis, MO, USA).

Cryptotanshinone (≥98% purity) was obtained from ChemFaces (Wuhan, China). Antibodies against SREBP-1c (H-160; sc-8984), PPARα (H-2; sc-398394), IkBα (H-4; sc-164), NFκB p65 (F-6; sc-8008p65), AMPK α1/2 (D-6; sc-74461), SIRT1 (H-300; sc-15404), and β-actin (C4; sc-47778) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Specific primary antibodies against p-ACC (Ser79; #3661s) and ACC (C83B10; #3676s) were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against CYP2E1 (ab28146) and pAMPK α1 (Thr172; PA5-17831) were purchased from Abcam (Cambridge, MA, USA) and Invitrogen (Rockford, IL, USA), respectively. The Nrf2 antibody (NBP1-32822) was obtained from Novus Biologicals (Centennial, CO, USA). Ethanol, Ex52735, and compound C were obtained from Sigma (St. Louis, MO, USA).