Log In Sign up
The largest database of trusted experimental protocols

Lago x optical imaging system

Manufactured by Spectral Instruments Imaging
Visit Supplier
Sourced in United States

The Lago X is an optical imaging system designed for high-performance, multi-modal imaging applications. It features a modular design that allows for the integration of a variety of imaging modalities, including fluorescence, bioluminescence, and radioisotopic imaging. The Lago X provides a versatile and scalable platform for a wide range of research and clinical applications.

Automatically generated - may contain errors

Lab products found in correlation

Ivis spectrum in vivo imaging system, by PerkinElmer (2 mentions) Rediject d luciferin ultra bioluminescent substrate, by PerkinElmer (2 mentions) Aura software, by Spectral Instruments Imaging (2 mentions) Living image software, by PerkinElmer (2 mentions) Xenolight d luciferin k salt bioluminescent substrate, by PerkinElmer (2 mentions) Ivisbrite d luciferin potassium salt bioluminescent substrate, by PerkinElmer (1 mentions) Aura imaging software, by Spectral Instruments Imaging (1 mentions) Xenolight d luciferin potassium salt, by PerkinElmer (1 mentions)

Close spelling variants of this product

Lago optical imaging system Lago X Imaging System Lago imaging system Lago X system Lago X

7 protocols using lago x optical imaging system

1

In Vivo Bioluminescent Imaging of Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Prior to imaging, the mice were i.p. injected with 100 μL of 30 mg/ml RediJect D-Luciferin Ultra bioluminescent substrate (PerkinElmer). Mice were then anesthetized with isoflurane and imaged within 20 minutes for bioluminescence using the in vivo imager. All the bioluminescence data, except for those shown in Supplementary Figures 7d and e, were collected using the IVIS Spectrum In Vivo Imaging System (PerkinElmer), and bioluminescent photon outputs were quantified using the Living Image Software (PerkinElmer). The bioluminescence data shown in Supplementary Figures 7d and e were collected using the Lago X optical imaging system (Spectral Instruments Imaging) and analyzed using Aura software (Spectral Instruments Imaging).
Zhong G., Wang H., He W., Li Y., Mou H., Tickner Z.J., Tran M.H., Ou T., Yin Y., Diao H, & Farzan M. (2019). A reversible RNA on-switch that controls gene expression of AAV-delivered therapeutics in vivo. Nature biotechnology, 38(2), 169-175.
+ Open protocol
+ Expand
2

Firefly Luciferin Optical Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
Firefly luciferin solution was prepared per the manufacturer’s instructions (PerkinElmer). Mice from all of the treatment groups, including control and CF33-Fluc-treated mice, received i.p. delivery of luciferin using the Lago X optical imaging system (Spectral Instruments Imaging, Tucson, AZ, USA) after 15 min of incubation.
Kim S.I., Chaurasiya S., Sivanandam V., Kang S., Park A.K., Lu J., Yang A., Zhang Z., Bagdasarian I.A., Woo Y., Morgan J.T., Yin Z., Fong Y, & Warner S.G. (2022). Priming stroma with a vitamin D analog to optimize viroimmunotherapy for pancreatic cancer. Molecular Therapy Oncolytics, 24, 864-872.
+ Open protocol
+ Expand
3

Peritoneal Tumor Burden Quantification

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Because caliper measurement is not feasible for measuring peritoneal tumor burden, we used bioluminescent imaging as a surrogate for tumor burden, as previously described.49 50 (link) All animals were imaged with bioluminescence for luciferase activity in the peritoneum to identify peritoneal tumor implants and growth after IP SNU-16-ffluc, and the tumor burden was quantified once a week after treatment. D-luciferin solution was prepared by dissolving 1 g of IVISbrite D-Luciferin Potassium Salt Bioluminescent Substrate (PerkinElmer, catalog#122799-5, Waltham, Massachusetts, USA) in 35 mL of PBS at 28.5 mg/mL concentration. IP delivery (200 µL/mouse) was performed in all groups, and the mice were imaged using Lago X optical imaging system (Spectral Instruments Imaging, Tucson, Arizona, USA). Bioluminescence imaging was analyzed using Aura V.64 software and presented as photons/second for regions of interest.41 (link)
Yang A., Zhang Z., Chaurasiya S., Park A.K., Jung A., Lu J., Kim S.I., Priceman S., Fong Y, & Woo Y. (2023). Development of the oncolytic virus, CF33, and its derivatives for peritoneal-directed treatment of gastric cancer peritoneal metastases. Journal for Immunotherapy of Cancer, 11(4), e006280.
+ Open protocol
+ Expand
4

Bioluminescence Imaging for Tumor Burden

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Each animal underwent bioluminescence imaging for luciferase activity of the tumor and was quantified to evaluate for tumor burden once a week and prior to euthanizing. D-luciferin solution was prepared by dissolving 1 g of XenoLight D-luciferin—K+ Salt Bioluminescent Substrate (PerkinElmer, Waltham, MA) in 35 mL of PBS at 28.5 mg/mL concentration. IP delivery (200 μL/mouse) was performed in all groups and the mice were imaged using Lago X optical imaging system (Spectral Instruments Imaging, Tucson, AZ). Bioluminescence imaging was analyzed using Aura64 software (11 (link)).
Woo Y., Zhang Z., Yang A., Chaurasiya S., Park A.K., Lu J., Kim S.I., Warner S.G., Von Hoff D, & Fong Y. (2020). Novel Chimeric Immuno-Oncolytic Virus CF33-hNIS-antiPDL1 for the Treatment of Pancreatic Cancer. Journal of the American College of Surgeons, 230(4), 709-717.
+ Open protocol
+ Expand
5

In Vivo Bioluminescent Imaging of Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Prior to imaging, the mice were i.p. injected with 100 μL of 30 mg/ml RediJect D-Luciferin Ultra bioluminescent substrate (PerkinElmer). Mice were then anesthetized with isoflurane and imaged within 20 minutes for bioluminescence using the in vivo imager. All the bioluminescence data, except for those shown in Supplementary Figures 7d and e, were collected using the IVIS Spectrum In Vivo Imaging System (PerkinElmer), and bioluminescent photon outputs were quantified using the Living Image Software (PerkinElmer). The bioluminescence data shown in Supplementary Figures 7d and e were collected using the Lago X optical imaging system (Spectral Instruments Imaging) and analyzed using Aura software (Spectral Instruments Imaging).
Zhong G., Wang H., He W., Li Y., Mou H., Tickner Z.J., Tran M.H., Ou T., Yin Y., Diao H, & Farzan M. (2019). A reversible RNA on-switch that controls gene expression of AAV-delivered therapeutics in vivo. Nature biotechnology, 38(2), 169-175.
+ Open protocol
+ Expand
6

Bioluminescence Imaging for Tumor Burden

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Each animal underwent bioluminescence imaging for luciferase activity of the tumor and was quantified to evaluate for tumor burden once per week. d-Luciferin solution was prepared by dissolving 1 g XenoLight d-luciferin-K + Salt Bioluminescent Substrate (PerkinElmer, catalog no. 122,799-5, Waltham, MA, USA) in 35 mL PBS at a 28.5-mg/mL concentration. Delivery i.p. (200 μL/mouse) was performed in all of the groups, and the mice were imaged using the Lago X optical imaging system (Spectral Instruments Imaging, Tucson, AZ, USA). Bioluminescence imaging was analyzed using Aura64 software (Spectral Instruments Imaging) and presented as photons/second for regions of interest.19 (link)
Zhang Z., Yang A., Chaurasiya S., Park A.K., Kim S.I., Lu J., Olafsen T., Warner S.G., Fong Y, & Woo Y. (2021). PET imaging and treatment of pancreatic cancer peritoneal carcinomatosis after subcutaneous intratumoral administration of a novel oncolytic virus, CF33-hNIS-antiPDL1. Molecular Therapy Oncolytics, 24, 331-339.
+ Open protocol
+ Expand
7

Adoptive CAR T-cell Therapy in Xenograft Mouse Model

Check if the same lab product or an alternative is used in the 5 most similar protocols
All animal experiments were performed under protocols approved by the City of Hope Institutional Animal Care and Use Committee (IACUC). Mice (6–8-week-old NSG mice) were intravenously injected with 5 × 105 Sup-B15 on day 5. Mice were treated with 1 × 106 CAR T cells intravenously. Tumor burden was monitored by live mice imaging using LagoX optical imaging system (Spectral Instruments Imaging, Tucson, AZ, USA). Mice were imaged by injecting XenoLight D-luciferin potassium salt (Perkin Elmer, Waltham, MA, USA) and analyzed on Aura Imaging Software (Spectral Instruments Imaging, Tucson, AZ, USA). Mice were retro-orbitally bled and upon euthanasia, bone marrow and blood were collected for flow cytometry.
Urak R., Gittins B., Soemardy C., Grepo N., Goldberg L., Maker M., Shevchenko G., Davis A., Li S., Scott T., Morris K.V., Forman S.J, & Wang X. (2023). Evaluation of the Elements of Short Hairpin RNAs in Developing shRNA-Containing CAR T Cells. Cancers, 15(10), 2848.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!