Prior to imaging, the mice were i.p. injected with 100 μL of 30 mg/ml RediJect D-Luciferin Ultra bioluminescent substrate (PerkinElmer). Mice were then anesthetized with isoflurane and imaged within 20 minutes for bioluminescence using the in vivo imager. All the bioluminescence data, except for those shown in Supplementary Figures 7d and e , were collected using the IVIS Spectrum In Vivo Imaging System (PerkinElmer), and bioluminescent photon outputs were quantified using the Living Image Software (PerkinElmer). The bioluminescence data shown in Supplementary Figures 7d and e were collected using the Lago X optical imaging system (Spectral Instruments Imaging) and analyzed using Aura software (Spectral Instruments Imaging).
Lago x optical imaging system
Manufactured by Spectral Instruments Imaging
Sourced in United States
The Lago X is an optical imaging system designed for high-performance, multi-modal imaging applications. It features a modular design that allows for the integration of a variety of imaging modalities, including fluorescence, bioluminescence, and radioisotopic imaging. The Lago X provides a versatile and scalable platform for a wide range of research and clinical applications.
Automatically generated - may contain errors
Lab products found in correlation
Ivis spectrum in vivo imaging system, by PerkinElmer (2 mentions)
Rediject d luciferin ultra bioluminescent substrate, by PerkinElmer (2 mentions)
Aura software, by Spectral Instruments Imaging (2 mentions)
Living image software, by PerkinElmer (2 mentions)
Xenolight d luciferin k salt bioluminescent substrate, by PerkinElmer (2 mentions)
Ivisbrite d luciferin potassium salt bioluminescent substrate, by PerkinElmer (1 mentions)
Aura imaging software, by Spectral Instruments Imaging (1 mentions)
Xenolight d luciferin potassium salt, by PerkinElmer (1 mentions)
7 protocols using lago x optical imaging system
1
In Vivo Bioluminescent Imaging of Mice
2
Firefly Luciferin Optical Imaging
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Firefly luciferin solution was prepared per the manufacturer’s instructions (PerkinElmer). Mice from all of the treatment groups, including control and CF33-Fluc-treated mice, received i.p. delivery of luciferin using the Lago X optical imaging system (Spectral Instruments Imaging, Tucson, AZ, USA) after 15 min of incubation.
3
Peritoneal Tumor Burden Quantification
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Because caliper measurement is not feasible for measuring peritoneal tumor burden, we used bioluminescent imaging as a surrogate for tumor burden, as previously described.49 50 (link) All animals were imaged with bioluminescence for luciferase activity in the peritoneum to identify peritoneal tumor implants and growth after IP SNU-16-ffluc, and the tumor burden was quantified once a week after treatment. D-luciferin solution was prepared by dissolving 1 g of IVISbrite D-Luciferin Potassium Salt Bioluminescent Substrate (PerkinElmer, catalog#122799-5, Waltham, Massachusetts, USA) in 35 mL of PBS at 28.5 mg/mL concentration. IP delivery (200 µL/mouse) was performed in all groups, and the mice were imaged using Lago X optical imaging system (Spectral Instruments Imaging, Tucson, Arizona, USA). Bioluminescence imaging was analyzed using Aura V.64 software and presented as photons/second for regions of interest.41 (link)
4
Bioluminescence Imaging for Tumor Burden
5
In Vivo Bioluminescent Imaging of Mice
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Prior to imaging, the mice were i.p. injected with 100 μL of 30 mg/ml RediJect D-Luciferin Ultra bioluminescent substrate (PerkinElmer). Mice were then anesthetized with isoflurane and imaged within 20 minutes for bioluminescence using the in vivo imager. All the bioluminescence data, except for those shown in Supplementary Figures 7d and e , were collected using the IVIS Spectrum In Vivo Imaging System (PerkinElmer), and bioluminescent photon outputs were quantified using the Living Image Software (PerkinElmer). The bioluminescence data shown in Supplementary Figures 7d and e were collected using the Lago X optical imaging system (Spectral Instruments Imaging) and analyzed using Aura software (Spectral Instruments Imaging).
6
Bioluminescence Imaging for Tumor Burden
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Each animal underwent bioluminescence imaging for luciferase activity of the tumor and was quantified to evaluate for tumor burden once per week. d -Luciferin solution was prepared by dissolving 1 g XenoLight d -luciferin-K + Salt Bioluminescent Substrate (PerkinElmer, catalog no. 122,799-5, Waltham, MA, USA) in 35 mL PBS at a 28.5-mg/mL concentration. Delivery i.p. (200 μL/mouse) was performed in all of the groups, and the mice were imaged using the Lago X optical imaging system (Spectral Instruments Imaging, Tucson, AZ, USA). Bioluminescence imaging was analyzed using Aura64 software (Spectral Instruments Imaging) and presented as photons/second for regions of interest.19 (link)
7
Adoptive CAR T-cell Therapy in Xenograft Mouse Model
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All animal experiments were performed under protocols approved by the City of Hope Institutional Animal Care and Use Committee (IACUC). Mice (6–8-week-old NSG mice) were intravenously injected with 5 × 105 Sup-B15 on day 5. Mice were treated with 1 × 106 CAR T cells intravenously. Tumor burden was monitored by live mice imaging using LagoX optical imaging system (Spectral Instruments Imaging, Tucson, AZ, USA). Mice were imaged by injecting XenoLight D-luciferin potassium salt (Perkin Elmer, Waltham, MA, USA) and analyzed on Aura Imaging Software (Spectral Instruments Imaging, Tucson, AZ, USA). Mice were retro-orbitally bled and upon euthanasia, bone marrow and blood were collected for flow cytometry.
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