Ysi 2300 stat plus glucose and lactate analyzer

Blood samples were collected at time 0 (fasted baseline) and 15, 30, 60, 90, 120, 180, 240, 300, and 360 minutes post beverage in ethylenediaminetetraacetic acid (EDTA)-containing tubes, gently inverted five times, and placed on ice until centrifugation. The blood was centrifuged at 3,200 rpm for 10 minutes at 4°C. The plasma from each EDTA-containing tube was pipetted into Eppendorf tubes and stored at −80°C for future analysis. Plasma samples were thawed from freeze at a later time, and plasma glucose and plasma lactate were measured in duplicate using the YSI 2300 Stat Plus™ Glucose and Lactate Analyzer (Yellow Springs Instruments).
Plasma samples were assayed for TAG and insulin concentrations at the UCH-CTRC Laboratory at the Medical Campus of the University of Colorado Denver. Plasma TAG and plasma insulin concentrations were analyzed at all time points, with the exception of 15 and 60 minutes following meal consumption. Insulin was measured utilizing a chemiluminescent immunoassay on a Beckman Access 2 analyzer (Beck-man Coulter, Inc.), with a reportable range between 1 and 300 μUI/mL. TAGs were measured using an enzymatic assay on a Beckman AU480 chemistry analyzer (Beckman Coulter, Inc.), with a reportable range between 15 and 950 mg/dL.