DMSO was purchased from American Bioanalytical (Natick, MA). Rosiglitazone (Rosi) was from Cayman Chemical (Ann Arbor, MI). Human insulin, dexamethasone, 3-isobutyl-1-methylxanthine (IBMX), LG100268 (LG268), and tributyltin (TBT) chloride were from Sigma-Aldrich (St. Louis, MO). All other reagents were from Thermo Fisher Scientific (Suwanee, GA) unless noted.
Rosiglitazone rosi
Manufactured by Cayman Chemical
Sourced in Macao, Germany
Rosiglitazone (Rosi) is a chemical compound used as a laboratory reagent. It functions as a peroxisome proliferator-activated receptor gamma (PPARγ) agonist.
Automatically generated - may contain errors
Lab products found in correlation
Dexamethasone (dex), by Merck Group (3 mentions)
Gw9662, by Cayman Chemical (2 mentions)
Human insulin, by Merck Group (1 mentions)
Propionate, by Merck Group (1 mentions)
Succinate, by Merck Group (1 mentions)
Butyrate, by Merck Group (1 mentions)
Pioglitazone pio, by Cayman Chemical (1 mentions)
Raw264.7 cell, by American Type Culture Collection (1 mentions)
Hek293 cell, by American Type Culture Collection (1 mentions)
Bovine serum albumin (bsa), by Thermo Fisher Scientific (1 mentions)
Mip 1α, by R&D Systems (1 mentions)
Puromycin, by Cayman Chemical (1 mentions)
Mip 2, by R&D Systems (1 mentions)
C57bl 6 mice, by Model Animal Research Center of Nanjing University (1 mentions)
Chip kit, by Merck Group (1 mentions)
Dual luciferase reporter assay system, by Promega (1 mentions)
Oxidized glutathione gssg assay kit, by Solarbio (1 mentions)
Superoxide dismutase sod assay kit, by Solarbio (1 mentions)
3 isobutyl 1 methylxanthine ibmx, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
11 protocols using rosiglitazone rosi
1
Adipocyte Differentiation Assay
2
Activation of PPARγ in HT-29 Cells
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The human epithelial cell lines HT-29 were obtained from the American Type Culture Collection (ATCC, Rockville, MD), HT-29 cells were grown in RPMI 1640 supplemented with 2 mM L-glutamine, 50 IU/mL penicillin, 50 μg/mL streptomycin and 10% heat-inactivated fetal calf serum (FCS) in a humidified 5% CO2 atmosphere at 37 °C. All culture media were supplied by Lonza. All agonists and inhibitors were dissolved in DMSO following the manufacturer’s recommendations. PPARγ activator TDZs: pioglitazone (Pio, 5 μM), rosiglitazone (Rosi, 10 μM), troglitazone (Tro, 5 μM) were from Cayman Chemicals and were used interchangeably due to their same level of activation on the used reporter system. MAPK kinase inhibitor: U0126 (MEK1/2) was purchased from Calbiochem. Butyrate was used at 2 mM except in the dose-response experiment where a range of concentrations from 0.5 to 8 mM was assessed for all the compounds tested (acetate, Butyrate, propionate, formate, lactate and succinate (Sigma).
3
Silencing POM121A/C in Cell Culture
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Chemicals were obtained from Merck (Darmstadt, Germany) if not stated otherwise. Antibodies (Abs) are listed in Table S1 . Rosiglitazone (rosi) was purchased from Cayman (Ann Arbor, MI). Control siRNA and siRNA against human POM121A/C transcripts were from Ambion Silencer® Select (s59624) (Thermofisher Scientific, Waltham, MA).
4
Murine Model of Inflammatory Response
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Pathogen-free male C57BL/6 mice that are 8–12 weeks old are obtained from Model Animal Research Center of Nanjing University (Nanjing, China), and all animal experiments are performed according to the protocol approved by Southeast University. RAW264.7 cells and HEK293 cells are purchased from American Type Culture Collection and cultured in DMEM supplemented with 10% fetal bovine serum (Gibico). Bovine serum albumin (BSA) and rabbit anti-BSA IgG (α-BSA) are purchased from Invitrogen and MP Biomedicals, respectively. ELISA kit for mouse albumin is purchased from Bethyl Laboratories. ELISA kits for TNF-α, MCP-1, MIP-1α and MIP-2 are purchased from R&D Systems. PPARγ antagonist GW9662, PPARγ agonist Rosiglitazone (ROSI) and puromycin are purchased from Cayman Chemical.
5
Rosi Regulates miR-21-5p and SFRP5
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Rosiglitazone (Rosi) was purchased from Cayman Chemical Company. miR-21-5p mimic, inhibitor and negative control (NC) were synthesized by Shanghai GenePharma Co., Ltd. Superoxide dismutase (SOD) assay kit, methane dicarboxylic aldehyde (MDA) assay kit, glutathione peroxidase (GSH) assay kit and oxidized glutathione (GSSG) assay kit were purchased from Beijing Solarbio Science & Technology Co., Ltd. miRNA Detection kit was purchased from GeneCopoeia, Inc. ChIP kit was from Merck KGaA. Dual-luciferase reporter assay system was from Promega Corporation. Human miR-21-5p promoter region or mutations in PPARγ binding sites were synthesized by Sangon Biotech Co., Ltd. The 3′-UTR of SFRP5 mRNA or mutant binding site was synthesized by Sangon Biotech Co., Ltd.
6
Evaluating PFAS Exposure Effects
7
Colon Cancer Cell Line PPAR-γ Agonist Assay
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The colon adenocarcinoma cell lines HT29 (ATCC HTB-38) and SW480 (ATCC CCL-228) were obtained directly from the American Tissue Culture Collection (Rockville, MD) and routinely cultivated under standard tissue culture conditions (humidified atmosphere of 95% air +5% CO2 at 37°C) using minimal essential medium (MEM) containing 10% fetal bovine serum, 100 U/ml penicillin, and 100 mg/ml streptomycin (PAA, Pasching, Austria). For experiments, the medium was replaced with serum-free MEM containing either vehicle control (DMSO) or indicated concentrations of the PPAR-γ agonists Ciglitazone (CIG), Troglitazone (TRO), and Rosiglitazone (ROSI) or the PPAR-γ antagonist GW9662 (all from Cayman Chemical, Ann Arbor, MI). The natural PPAR-γ agonists Prostaglandin J2 (PGJ2) was purchased from Sigma (St. Louis, MO) and used as a positive control. All cell culture experiments were performed independently three times from different passages.
8
Signaling Pathway Profiling of Cell Lines
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Chemicals were purchased from Merck (Darmstadt, Germany) or Sigma (Heidelberg, Germany). Antibodies were MTMR7 (#121222, Abcam, Cambridge, UK), phospho-AKT-S473 (#4060), phospho-AKT-T308 (#13038), AKT (#9272), phospho-S6 ribosomal protein (#4858), S6 ribosomal protein (#2217), phospho-ERK1/2 (#4370) (all from Cell Signaling, Danvers, MA), ERK2 (sc-154), HSP90 (sc-7947) (both from Santa Cruz Biotech., CA) and beta-actin (A1978, Sigma). Rosiglitazone (rosi) was obtained from Cayman Chemical Company (Ann Arbor, Michigan). 12-O-tetradecanoylphorbol-13-acetate (TPA) and human recombinant IGF1/2 were from Sigma, insulin and EGF from Roche Diagnostics GmbH, Mannheim, Germany.
9
Screening Compounds for Nuclear Receptor Modulators
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Dexamethasone, isobutylmethylxanthine, Nile red, Hoechst 33342, HX531, LG100268 (LG), 2,4-di-tert-butylphenol (2,4-DTBP), 2,6-di-tert-butylphenol (2,6-DTBP), 2,4,6-tri-tert-butylphenol (2,4,6-TTBP), 1,3-di-tert-butylbenzene (1,3-DTBB), 1,3,5-tri-tert-butylbenzene (1,3,5-TTBB), LG100268, GW3065, T3, and TTNPB were purchased from Sigma-Aldrich. CD3254 was purchased from Tocris Bioscience. Rosiglitazone (ROSI) was purchased from Cayman Chemical Company. T0070907 was from Enzo Life Sciences. Dimethylsulfoxide (DMSO) was purchased from Thermo Fisher Scientific.
10
In Vitro Chemical Bioassay Protocol
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