In some animals, the retina was imaged using the scanner 3D OCT-2000 (Topcon, Tokyo, Japan), allowing a complete assessment of the area around the MEA. Each acquisition combined both OCT and fundus imaging. The OCT has an axial resolution of 5 μm. The superluminescent diode light source used is centered at 840 nm with a bandwidth of 50 nm adapted for retinal imaging. The focus was adjusted manually on the retina above the MEA. The analysis was performed using 3D OCT-2000 software (Topcon, Tokyo, Japan) and consisted in the localization of the MEA on the corresponding B-Scan cross-sections. For fundus images, the OCT was combined with a camera (Nikon R_D90, Nikon Imaging Japan Inc.) that uses a white light flash with green filter, allowing color or red-free acquisitions. An additional +20 D magnifying lens was used to enlarge the field of view of the apparatus originally designed for measurements on humans. Note that the use of this lens induced optical artifacts in fundus (Figure 1C top row, halos of light). Finally, the positions of the implant and of the optic disk were back projected onto the visual stimulation screen using an ophthalmoscope coupled with a laser.
3d oct 2000
Manufactured by Topcon
Sourced in Japan, United States, Germany
The 3D OCT-2000 is a high-resolution optical coherence tomography (OCT) system designed for clinical use. It provides detailed, three-dimensional imaging of the anterior and posterior segments of the eye. The device utilizes non-invasive near-infrared light to capture cross-sectional images of the eye's structures, enabling healthcare professionals to assess and monitor various ocular conditions.
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Lab products found in correlation
Hem 759e, by Omron (4 mentions)
Iol master, by Zeiss (4 mentions)
Rs 3000, by Nidek (3 mentions)
Trc 50dx, by Topcon (3 mentions)
Dri oct triton, by Topcon (3 mentions)
Spectralis, by Heidelberg Engineering (3 mentions)
Oa 2000, by Tomey (2 mentions)
Trc nw7sf, by Topcon (2 mentions)
Heidelberg spectralis, by Heidelberg Engineering (2 mentions)
Spss 19, by IBM (1 mentions)
Iolmaster, by Topcon (1 mentions)
Nonmyd wx, by Kowa (1 mentions)
Dri oct, by Topcon (1 mentions)
Jgstkdiscanalysissoft, by Topcon (1 mentions)
Spectralis hra oct, by Heidelberg Engineering (1 mentions)
Vx 10 fundus camera, by Kowa (1 mentions)
3d oct 1000, by Topcon (1 mentions)
Humphrey field analyzer, by Zeiss (1 mentions)
Humphrey field analyzer hfa, by Zeiss (1 mentions)
Maestro2, by Topcon (1 mentions)
79 protocols using 3d oct 2000
1
Retinal Imaging with 3D OCT-2000
2
Standardized Methodology for Choroidal Thickness Measurement
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OCT scans were conducted in two different cities using the same device (3D OCT-2000® Topcon, Topcon Inc., Tokyo, Japan) and were performed within the same time (08:00 am to 10:00 am) to avoid diurnal changes in choroidal thickness. All measurements were taken from the myoticpupilla and were performed in a semi-illuminated operating room.
During the same visit, a high-resolution (l = 840 nm, 27,000 A-scans/5 mm axial resolution) 6-mm single-line horizontal scanning (centering on the fovea) was conducted using an SD-OCT platform (3D OCT-2000® Topcon, Topcon Inc., Tokyo, Japan) operating in the enhanced depth imaging (EDI) mode. This device also provided automated central macular thickness (CMT) measurements.[Fig. 1 ].
All OCT measurements were performed by two experienced technicians during a single patient visit. For each eye, CTs were measured three times at a 20-min interval and then the mean values were recorded. The eyes in which there is a difference of >5% among the three measurements were excluded. The choroidal thickness, the extent of which starts from the outer edge of the retinal pigment epithelium (RPE) to the inner scleral border, was manually measured [Fig. 2 ]. The measurements were performed by two independent observers (Dr. M.G. and Dr. S.K). Interobserver differences were within 10% of the mean values.
During the same visit, a high-resolution (l = 840 nm, 27,000 A-scans/5 mm axial resolution) 6-mm single-line horizontal scanning (centering on the fovea) was conducted using an SD-OCT platform (3D OCT-2000® Topcon, Topcon Inc., Tokyo, Japan) operating in the enhanced depth imaging (EDI) mode. This device also provided automated central macular thickness (CMT) measurements.[
All OCT measurements were performed by two experienced technicians during a single patient visit. For each eye, CTs were measured three times at a 20-min interval and then the mean values were recorded. The eyes in which there is a difference of >5% among the three measurements were excluded. The choroidal thickness, the extent of which starts from the outer edge of the retinal pigment epithelium (RPE) to the inner scleral border, was manually measured [
3
Macular Retinal Layer Analysis with 3D OCT
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We measured macular RNFLT and GCCT with 3D OCT-2000 software (version 8.00; Topcon Corporation, Tokyo, Japan). After obtaining macular cube scans (7 × 7 square mm, corresponding to a 10-degree square area of the retina in the macula) centered on the fovea, the embedded 3D OCT program was used to calculate the thickness of each layer automatically.
4
Optic Nerve Fiber Layer Thickness
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CpRNFLT was measured with 3D OCT-2000 software (version 8.00; Topcon Corporation, Tokyo, Japan). Twelve circular scans (3.46 mm in diameter) were taken, centered on the optic disc. The supplied software then determined cpRNFLT over the entire circumference of the optic disc. Images with quality less than 70 were excluded.
5
Macular Maps from Optical Coherence Tomography
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Maps of mRNFLT, mGCIPLT and cpRNFLT were derived from macular cube scans of a 6 x 6 mm square area that corresponded to the central 20 degrees. The maps were centered on the fovea and captured with 3D OCT-2000 software (ver. 8.00, Topcon Corporation, Tokyo, Japan). A 10 x 10 grid comprising 100 points was laid over the maps for the analysis. Scans were excluded if the image quality was less than 70 or if segmentation in the images was inaccurate. Every scan was manually checked for segmentation errors and was excluded if errors were found. Images that were not accurately centered on the macula were manually adjusted.
6
Retinal Layer Thickness Analysis
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CpRNFLT, mRNFLT, and GCCT were determined with 3D OCT-2000 software (version 8.00; Topcon Inc.). After obtaining circle scans and macular cube scans (in a 7 × 7 mm area corresponding to 10-degree square area of the macula) centered on the fovea, the software automatically calculated the thickness of each layer.
7
Retinal Layer Segmentation Protocol
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The CPB was defined in this study as a 1.5 × 9.0 mm rectangular area centered between the optic nerve disc and the macula, aligned perpendicularly to the nerve fibers. At either end of the scan area, a 1.5 × 1.2 mm area in which the retinal layers could not be reliably segmented was discarded. The remaining 1.5 × 6.6 mm area was divided lengthwise into three 1.5 × 2.2 mm sections, representing the upper, middle, and lower CPB. Analysis of the CPB used 3D OCT images, obtained with the 3D OCT-2000 (Topcon Corporation, Tokyo, Japan) device. Each image was constructed from 64 B-scan images (pixel dimensions: 512 × 885, grayscale levels: 256) with depth and lateral resolutions of 6 μm and 20 μm, respectively. Layer segmentation was performed with newly developed software (Topcon). The RNFLT and GCCT of the CPB were measured with automatic analysis software developed by the Graduate School of Science and Engineering, Yamagata University. This software was equipped with a registration system (using a fast registration algorithm for the 3D OCT images based on en-face projection images) to ensure the reliability and reproducibility of the clinical data.
8
Comprehensive Evaluation of Aphakic Patients
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The ethical committee of the hospital approved the study. It was hospital-based descriptive type of observational study which included 50 eyes of fifty patients. All aphakic patients above 12 years who were ready to give consent were included in the study. Exclusion criteria included patients with corneal opacity, retinal disorder, optic atrophy, bleeding disorder, pregnancy, and those who were unwilling to give consent. Preoperative and postoperative visual acuity, slit lamp and fundus examination, applanation tonometry, keratometry, biometry Carl Zeiss Meditec IOL Master, and optical coherence tomography (OCT) (Topcon 3D OCT 2000) were done for extensive evaluation of anterior and posterior segment.
Statistical analysis was done with the help of IBM SPSS 19.0 software. Qualitative data were summarized in the form of proportion. Quantitative data were summarized in the form of mean and standard deviation (SD). The significance of difference in proportion was measured by Chi-square test. The significance of difference in mean was measured by unpaired t-test or ANOVA whichever is appropriate. P < 0.05 was considered as statistically significant.
Statistical analysis was done with the help of IBM SPSS 19.0 software. Qualitative data were summarized in the form of proportion. Quantitative data were summarized in the form of mean and standard deviation (SD). The significance of difference in proportion was measured by Chi-square test. The significance of difference in mean was measured by unpaired t-test or ANOVA whichever is appropriate. P < 0.05 was considered as statistically significant.
9
Optimized Retinal Imaging Protocols
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A sdOCT machine (3D OCT-2000, Topcon) and the following three scan protocols were used: 6.0×6.0 mm 3D disc (512 A-scans by 128 B-scans); 6.0×6.0 mm 3D macula (512 A-scans by 128 B-scans); and 3.4 mm dia. circle (average of 50 scans; 1024 A-scans). The circle protocol involved averaging 50 individual scans and thus produced an image of relatively high-quality. (Note that shadowgrams (see inset in panel E of figures 3 (green arrow), 5 and 6 ) can be examined for artefacts such as excessive eye movements, blinks, etc.)
10
Ocular Blood Flow and Retinal Nerve Fiber Layer
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The corresponding en face image (the 6 mm × 6 mm image displaying the same segment located within 100 μm of the inner limiting membrane as in the above angioflow image) was then used to identify the area of the RNFL defect. The angle of the RNFL defect was measured using the same method as that used to determine the disappearance angle of the RPCs.
Segmentation of the peripapillary area was performed based on cpRNFL thickness measured using the OCT angiography Angio Disc software (Fig 2A ) and the 3D-OCT 2000 (Topcon) optic disc mapping function (Fig 2B ). Exclusion criteria for 3D-OCT 2000 images were unclear optic discs or image quality ≤40.
Segmentation of the peripapillary area was performed based on cpRNFL thickness measured using the OCT angiography Angio Disc software (
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