DIG-labeled riboprobes were synthesized using a DIG RNA labeling kit (Roche) and were used on 20μm transverse sections. Template for Ndfip1 probe was amplified from a mouse DRG cDNA library and Ndfip2 probe was amplified from mouse Ndfip2 ORF clone (Origene #MR202968). mRNA signal was visualized using BCIP/NBT and AP-conjugated anti-DIG antibody. Primers used to amplify cDNA were: Ndfip1 (5′- AGAACGTCTCAGCGTCGG –3′ and 5′-CAGGAAGCCTTTGCCAGA –3′) and Ndfip2 (5′-ATGCGCGTCCGCGCCGAGCAT –3′ and 5′-CTCGTCCTATGTGCAGCCGCCATAC –3′).
Ndfip2 orf clone
Manufactured by OriGene
Ndfip2 ORF clone is a laboratory product that contains the open reading frame (ORF) of the Ndfip2 gene. The Ndfip2 gene encodes a protein that is believed to play a role in protein trafficking and ubiquitination processes within cells. This product is intended for use in research applications, such as gene expression studies or protein interaction experiments, where the Ndfip2 gene or its encoded protein may be of interest.
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Lab products found in correlation
2 protocols using ndfip2 orf clone
1
In Situ Hybridization of Ndfip1 and Ndfip2
2
In Situ Hybridization of Ndfip1 and Ndfip2
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