The acylglycerol composition of raw SSBO and reaction products were determined by a normal phase HPLC (LC-15, Shimadzu, Japan) equipped with a refractive index detector. The samples were dissolved into the mobile phase (21:1:0.004, v/v/v, n-hexane/isopropyl alcohol/formic acid), and then, eluted via the gradient elution of the mobile phase. The chromatographic conditions were set as follows: column temperature, 30°C; flow rate, 1 mL/min; sample injection volume, 10 μl. The content of each lipid class was calculated by peak-areas percentages. The DAG content was calculated based on the sum peak area of 1,3-DAG and 1,2-DAG concerning the total peak areas. The conversions of EE or FFA were calculated as the glycerolysis of EE amount or the esterified FFA amount to the initial EE or FFA amount.
Lc 15
Manufactured by Shimadzu
Sourced in Japan, China
The LC-15 is a high-performance liquid chromatography (HPLC) system manufactured by Shimadzu. It is designed for the separation, identification, and quantification of various chemical compounds in a sample. The LC-15 utilizes a liquid mobile phase to carry the sample through a stationary phase, typically a column, to achieve the desired separation of analytes.
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Lab products found in correlation
4 protocols using lc 15
1
HPLC Analysis of Acylglycerol Composition
2
Automated Radiosynthesis of 18F-FAPI
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Radiosynthesis was as follows[32 (link), 33 (link)]: 18 F-FAPI was automatically synthesized with modified AllinOne module (Trasis, Ans, Belgium). 18F-was attained on-site on a Sumitomo HM-10 cyclotron system (Sumitomo Heavy Industries, Tokyo, Japan), where [18O]H2O was irradiated with 10 MeV protons, and reacted with 0.15 mg NOTA-FAPI-04 (Paite Biotech, Beijing, China) (130 °C, 8 min, pH 4.0). An HLB cartridge (Waters Corporation) was used to collect the purified products. Quality of the final 18F-FAPI product was tested by HPLC (Shimadzu LC-15, Suzhou, China). Radiochemical purity was > 95%. Appearance, color, pH, and other quality controls were performed according to the current pharmacopoeias.
3
HPLC Analysis of Liquid Phase
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The liquid phase composition of the solution was determined by Japanese Shimadzu LC15 high performance liquid chromatography in 282 nm.
4
Quantitative Analysis of Tea Polyphenols
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The appropriate amounts of four standard substances (EGCG, EGC, EC and ECG), were accurately weighed and dissolved in 20% methanol (v/v). The tea polyphenol samples were prepared with 20% methanol and injected into the HPLC system for analysis after filtering through 0.22 µm syringe filters.
The analysis was carried out using an LC 15 (Shimadzu Corporation) for the chromatographic determination. The sample was injected into a C18 analytical column (4.6 mm I.D x 150 mm, 3.0 µm particle size; Waters Corporation). The mobile phase was 0.1% formic acid solution (solvent A) and methanol (solvent B). The Diode-Array Detection acquisition wavelength was set at 275 nm. The gradient elution programmer was as follows: ~0-2 min, 2% B; ~2-13 min, 2-90% B; ~13-16 min, 100%. The flow rate was of 0.3 ml/min, and the column temperature was set at 40˚C. The injection volume was 5 µl.
The analysis was carried out using an LC 15 (Shimadzu Corporation) for the chromatographic determination. The sample was injected into a C18 analytical column (4.6 mm I.D x 150 mm, 3.0 µm particle size; Waters Corporation). The mobile phase was 0.1% formic acid solution (solvent A) and methanol (solvent B). The Diode-Array Detection acquisition wavelength was set at 275 nm. The gradient elution programmer was as follows: ~0-2 min, 2% B; ~2-13 min, 2-90% B; ~13-16 min, 100%. The flow rate was of 0.3 ml/min, and the column temperature was set at 40˚C. The injection volume was 5 µl.
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