Twenty set of lip print patterns along with their corresponding clinical photographs (using Sony Cybershot, 7.2 MPx) were obtained on bond paper fixed with cardboard using dark coloured lipstick, following guidelines given by Bindal et al.,(2009).[5 ] Numerical coding of photographs and patterns was done [Figures 1 and 2 ].
Cybershot
Manufactured by Sony
Sourced in Japan
The Cybershot is a digital camera line produced by Sony. It is designed to capture high-quality images and video. The Cybershot features a built-in lens, image sensor, and storage media.
Automatically generated - may contain errors
Lab products found in correlation
Stereo lumar v12 microscope, by Zeiss (1 mentions)
Mitomycin, by Merck Group (1 mentions)
Axioplan, by Zeiss (1 mentions)
Ff01 572 28 25, by IDEX Corporation (1 mentions)
Axiphot microscope, by Sony (1 mentions)
Fluoroshield solution with dapi, by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by LGC (1 mentions)
Dsc w70, by Sony (1 mentions)
12 well plates, by Celltreat (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Entelan, by Merck Group (1 mentions)
Nis elements imaging software, by Nikon (1 mentions)
22 protocols using cybershot
1
Lip Print Pattern Identification
2
Carmine Alum Staining of Mammary Glands
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As previously described [23] , the left inguinal mammary glands were excised, spread on glass slides and fixed in Carnoy's fixative (100% EtOH, chloroform, glacial acetic acid; 6∶3∶1) for 4 h at room temperature. Mammary glands were washed in 70% EtOH for 15 min, gradually rehydrated in water, and stained in carmine alum (2% carmine and 5% aluminum potassium sulfate in water) overnight at room temperature. Tissues were then gradually dehydrated through serial ethanol baths and cleared in xylene overnight. Mammary glands were kept in methyl salicylate until images were captured with a numeric camera (Cybershot, Sony) and a SteREOLumar V12 microscope (Zeiss) (N≥4).
3
Standardized Wound Healing Evaluation Methodology
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The wound healing process was evaluated using photographs acquired with a Sony Cyber Shot® digital camera (model DSC-S950S, 10 MP, 4× optical zoom) and an appropriate tripod to maintain an equal distance in all images. The photographs were taken daily by the same evaluator. When taking the photos, the animals were first placed in an acrylic box and anesthetized with isoflurane (BioChimico). The images were digitalized, and the wound area was measured using the Image J software (National Institutes of Health, Bethesda, MD, USA). The wound retraction is expressed as a percentage (%), measured by the following mathematical formula: (initial wound area)–(daily wound area) × 100 (home area).
Scar tissue was collected on days 6, 9, and 19 after injury from different groups of animals (five animals each). After collecting tissue, the animals were euthanized using a thiopental overdose and subsequent cervical dislocation. The tissue was stored at -80°C for future molecular and cellular evaluation.
During the experimental period, the mice were evaluated for motor activity, food acceptance, wound appearance (presence/absence of exudate), and death. Data were recorded for each mouse until the day of euthanasia, which was performed using a thiopental overdose and subsequent cervical dislocation.
Scar tissue was collected on days 6, 9, and 19 after injury from different groups of animals (five animals each). After collecting tissue, the animals were euthanized using a thiopental overdose and subsequent cervical dislocation. The tissue was stored at -80°C for future molecular and cellular evaluation.
During the experimental period, the mice were evaluated for motor activity, food acceptance, wound appearance (presence/absence of exudate), and death. Data were recorded for each mouse until the day of euthanasia, which was performed using a thiopental overdose and subsequent cervical dislocation.
4
Wound Healing Assay for SK-MEL-28 Cells
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In 24-well plates, 1 × 105/mL of SK-MEL-28 cells were seeded and cultured at 37 °C, 5% CO2 for 24 h. After reaching 90–100% confluence, 10 µg/mL of mitomycin (Sigma-Aldrich, St. Louis, MO, USA) was added 15 min before scraping. Using a P200, a risk was created in the center of the well, and a picture of the initial moment was taken (t = 0 h) with a camera (Sony Cyber-Shot) coupled to an inverted optical microscope. The medium was removed and the cells were treated with the respective drugs: 12.5, 25, 50, and 100 µM of CrataBL; 50, 100, and 200 µM pep. 26; 50, 100, and 200 µM pep. 27; and 2 µM of vemurafenib in each treatment. After 24 h, new photos were taken, as well as after 48 h. The size of the gap was quantified using the Image J program (ij153-win-java8), and graphs were plotted using GraphPad Prism 7 program [54 (link)]. The experiment was performed at least thrice.
5
GFP Expression in Transfected Embryos
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GFP expression in the transfected embryos in day 3 (72 h post-fertilization) was observed under fluorescent microscope (Zeiss Germany Axioplan). Microphotographs were taken using a digital camera (Sony, Cyber-Shot). The transfection efficiency was calculated as the percentage of fluorescent embryos out of the total number of embryos.
6
Evaluating Emergency Care Training
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The three examiners involved in the rating of participants’ actions are duly certified general surgeons with more than 5 years’ experience in emergency care. The examiners rated the participants’ actions based on the sequence of steps on the 10-item checklist for practical evaluation (S2 Checklist ). Points were given for actions performed correctly in sequence, and for the effectiveness and quality of chest compressions.
The manikin used in the simulation had a “clicker” feature that signalled the correct compression depth of 5 cm. For the purposes of this study, a chest compression was considered valid if the compression produced the audible feedback (“click”) indicating that the correct depth of 5 cm was achieved. The examiner gave 1 point for correct overall performance if compression produced an audible click in 50–75% of well-executed attempts, and 0 (zero) if there was an audible click in <50% of attempts. A video camera (Sony Cyber-shot, model DSC-HX5V, 2010) was used to record the simulated scenarios, and the recordings were later checked if there was any doubt about the actions of the participants.
The main outcome was the mean performance score of students on each (theoretical and practical) test for each study arm.
The manikin used in the simulation had a “clicker” feature that signalled the correct compression depth of 5 cm. For the purposes of this study, a chest compression was considered valid if the compression produced the audible feedback (“click”) indicating that the correct depth of 5 cm was achieved. The examiner gave 1 point for correct overall performance if compression produced an audible click in 50–75% of well-executed attempts, and 0 (zero) if there was an audible click in <50% of attempts. A video camera (Sony Cyber-shot, model DSC-HX5V, 2010) was used to record the simulated scenarios, and the recordings were later checked if there was any doubt about the actions of the participants.
The main outcome was the mean performance score of students on each (theoretical and practical) test for each study arm.
7
Whole Mount Mammary Gland Staining
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Whole mounts were performed similar to how we have previously described [61 (link)]. Briefly, mammary glands were dissected and flattened onto a glass slide before being placed into Carnoy's fixative (100% EtOH, chloroform, glacial acetic acid; 6:3:1) overnight at 4°C. Glands were immersed in 70% ethanol for 15 min and then transferred to descending concentrations of ethanol before being placed in carmine alum stain overnight at room temperature. Glands were put through increasing concentrations of ethanol and into xylene overnight. Glands were then placed in methyl salicylate for long term storage. Images were captured using a numeric camera (Sony Cybershot).
8
LAMP Reaction Imaging in Microfluidics
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Endpoint images of the LAMP reaction within the microfluidic card were captured using either a cell phone camera (Galaxy Note II, Samsung), or a digital camera (Cybershot, Sony). SYTO intercalating dye was excited using a 530 nm green LED (05027-PM12, LED Supply). A 572 ± 20 nm bandpass filter (FF01-572/28-25, Semrock) was fixed to capture emission signals from wells. For experiments with 40 % blood, a 0.25 megapixel monochrome CCD camera (MEADE DSI Pro, Irvine, CA, 1.0 s exposure time) was necessary to capture increased signal from an amplification event.
9
Anatomical Features of Pod DZ
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Anatomical features of pod DZ were observed in 30 random F2 plants and five F2:3 progenies from 20 F2 plants selected on the basis of their rupture energy (10 each with low RE and high RE). Pods were collected at 35–40 days after anthesis. Hand sections were prepared from one cm from the pedicel end of the pod. Fresh sections were observed for autofluorescence using a fluorescence microscope at the Charles Sturt University, Wagga Wagga. Photographs were taken using a Zeiss Axiphot microscope fitted with a Sony Cyber-shot digital camera.
10
Collagen Construct Area Analysis
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Each collagen construct was photographed at 24 h intervals; images being captured using a digital camera (Cybershot; Sony Tokyo, Japan) and analysed using software package ImageJ™ (National Institutes of Health, Bethesda, MD, USA). This software was used to calculate the area of the upper surface of each FPCM. Each area was measured three times and a mean calculated. Measurements were used to calculate the percentage reduction in area of each FPCM throughout the experiment.
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