Ultimate capillary lc system

Manufactured by Thermo Fisher Scientific

The Ultimate Capillary LC system is a high-performance liquid chromatography (HPLC) instrument designed for analytical and preparative separation of complex samples. The system features a compact design and is capable of operating at low flow rates, making it suitable for applications that require high resolution and sensitivity.

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Lab products found in correlation

Ltq orbitrap mass analyzer, by Thermo Fisher Scientific (1 mentions)

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Ultimate 3000 capillary LC system (2 citations)

2 protocols using ultimate capillary lc system

Phosphorylation Site Identification of METTL3

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To identify phosphorylation sites of METTL3, METTL3 precipitated from 293T cells co-transfected with myc-METTL3 and B-Raf V600E was analyzed by Coomassie Blue staining. The protein band corresponding to METTL3 was excised and subjected to in-gel digestion with tryspin and chymotrypsin. Samples were analyzed by Ultimate Capillary LC system (Dionex) directly coupled to LTQ Orbitrap Mass Analyzer (Thermo Scientific) using the TopTenTM method. The data were searched on MASCOT (MassMatrix) against the human Swiss-Prot database. All the identified phospho-peptides were further confirmed by manually checking the results.

Sun H.L., Zhu A.C., Gao Y., Terajima H., Fei Q., Liu S., Zhang L., Zhang Z., Harada B.T., He Y.Y., Bissonnette M.B., Hung M.C, & He C. (2020). Stabilization of ERK-Phosphorylated METTL3 by USP5 Increases m6A Methylation. Molecular cell, 80(4), 633-647.e7.

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Phosphorylation Sites Identification of AGO2

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To identify phosphorylation sites of AGO2, Flag‐AGO2 precipitated from 293T cells co‐transfected with TBK1 was analyzed by Coomassie Blue staining. The protein band corresponding to AGO2 was excised and subjected to in‐gel digestion with tryspin. Samples were analyzed by Ultimate Capillary LC system (Dionex) directly coupled to LTQ Orbitrap Mass Analyzer using the TopTenTM method. The data was searched on MASCOT (MassMatrix) against the human Swiss‐Prot database. All the identified phospho‐peptides were further confirmed by manually checking the results.

Zhao X., Cao Y., Lu R., Zhou Z., Huang C., Li L., Huang J., Chen R., Wang Y., Huang J., Cheng J., Zheng J., Fu Y, & Yu J. (2024). Phosphorylation of AGO2 by TBK1 Promotes the Formation of Oncogenic miRISC in NSCLC. Advanced Science, 11(15), 2305541.

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