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The MUM-1 is a laboratory equipment product manufactured by Santa Cruz Biotechnology. It is designed for use in scientific research and analysis. The core function of the MUM-1 is to perform specific tasks related to the handling and processing of biological samples. No further details on the intended use or capabilities of the product can be provided in an unbiased and factual manner.

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2 protocols using mum 1

1

Immunohistochemical Analysis of Lymphoma

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Tissue samples were fixed in 10% formalin and embedded in paraffin. Then, 4‐μm thick sections were cut and stained with hematoxylin and eosin. Monoclonal antibodies against the following molecules were used for immunohistochemical study: L26/CD20, BCL‐2, BCL‐6 (DAKO), MUM‐1 (Santa Cruz Biotechnologies), CD3, CD5, CD10 (Novocastra Laboratories), and PD‐L1 (antibody SP142, Spring Bioscience). The antibodies were applied after sections were heated in a microwave oven for antigen retrieval. Tissue samples were considered positive for the expression of proteins, when more than 30% of tumor cells showed positive staining with a specific antibody.
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2

Immunohistochemical Analysis of Murine Tissues

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Tissues were fixed in 4% Paraformaldehyde overnight and embedded in paraffin according to standard procedures. 5μm sections were either stained with Hematoxilin&Eosin or with the following antibodies: CD45R/B220 (ab64100, Abcam), CD3 (ab5690, Abcam), Ki-67 (RM-9106-S1, Thermo Scientific), IgG (BA2000, Vector), BRAF (sc-9002, Santa Cruz), pERK (4373, Cell Signaling), Bcl-6 (5650, Cell Signaling), and Mum1 (sc-6059, Santa Cruz). Organs from at least 5 mice from different litters were used for all stainings.
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