Tris hcl buffer ph 7.2

Materials for the preparation of the hydrogels, acrylamide (AAm, 40% w/v), N,N′-methylenebisacrylamide (Bis, 2% w/v), alginic acid (sodium salt, low viscosity), ammonium persulfate (APS), N,N,N′,N′-tetramethylethylenediamine (TEMED), and calcium chloride (CaCl₂) were purchased from Sigma Aldrich (Saint Louis, MO, USA), and agarose (low melting) was purchased from Thermo Fisher Scientific (Waltham, MA, USA), and used as received. Tris-HCl buffer (pH 7.2) was purchased from Life Technologies (Carlsbad, CA, USA) and binzil silica nanoparticle colloid solution with a mean particle size of 4 nm was a gift from AkzoNobel Pulp and Performance Chemicals Inc. (Marietta, GA, USA). 5-FU was purchased from Sigma (Saint Louis, MO, USA). Human U-87 glioblastoma cells were obtained from ATCC (Manassas, VA, USA), Dulbecco’s modified Eagle medium (DMEM) from Mediatech (Manassas, VA, USA), fetal bovine serum and penicillin-streptomycin from Invitrogen (Carlsbad, CA, USA), and sodium pyruvate, MEM non-essential amino acids and GlutaMax from Life Technologies (Carlsbad, CA, USA). WST cell proliferation assay kit was purchased from Dojindo Molecular Technologies (Rockville, MD, USA).

Materials for preparation of the hydrogels, acrylamide (40% w/v), acrylic acid (sodium salt), n,n′-methylenebis(acrylamide) (2% w/v), alginic acid (sodium salt, low viscosity), ammonium persulfate (APS), n,n,n′,n′-tetramethylethylenediamine (TEMED), and calcium chloride (CaCl₂) were purchased from Sigma Aldrich (Saint Louis, MO, USA), and agarose (low melting) was purchased from Thermo Fisher Scientific (Waltham, MA, USA), and used as received. Tris–HCl buffer (pH 7.2) was obtained from Life Technologies (Carlsbad, CA, USA) and binzil silica nanoparticle colloid solution with mean particle size of 4 nm was obtained as a gift from AkzoNobel Pulp and Performance Chemicals Inc. (Marietta, GA, USA).

All of the materials for the preparation of the single network and IPN hydrogels are as follows: acrylamide (AAm, 40% w/v), N,N′-methylenebis(acrylamide) (Bis, 2% w/v), ammonium persulfate (APS), N,N,N′,N′-tetramethyl ethylenediamine (TEMED), and low- and high-molecular weight dextran (100 kDa and 500 kDa) were purchased from Sigma Aldrich (St. Louis, MO, USA) and used as received. A Binzil silica nanoparticle (SiNP) colloid solution with a mean particle size of 4 nm was obtained as a gift from AkzoNobel Pulp and Performance Chemicals Inc. (Marietta, GA, USA). Tris–HCl buffer (pH 7.2) was purchased from Life Technologies (Carlsbad, CA, USA).