Mda mb 231

MDA-MB-453, MCF-7, BT474, ZR-75-1, MDA-MB-231, HCC1806, and MCF-10A were purchased from American Type Culture Collection (ATCC), while SUM1315 was friendly and generously obtained from Stephen Ethier (University of Michigan, AnnArbor, MI, USA). MCF-10A, MDA-MB-453, MCF-7, BT474, ZR-75-1, MDA-MB-231 and SUM1315 cells lines were cultured in Dulbecco’s modified eagle medium (DMEM) (Wisent, China), and HCC1806 cell line was cultured in RPMI 1640 (Wisent, China). 10% fetal bovine serum, 100 mg/ml streptomycin and 100 U/ml penicillin were added into DMEM or RPMI 1640.

The human adipose-derived mesenchymal stem/stromal cells (ADMSC) and TNBC-derived cell line MDA-MB-231 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). ADMSC were grown in Mesenchymal Stem Cell Basal Medium (ATCC, PCS-500-030) and supplemented with Mesenchymal Stem Cell Growth Kit—Low Serum (ATCC, PCS-500-040). They were further reported to have the capacity to undergo adipogenesis [27 (link)]. MDA-MB-231 were grown in EMEM Medium (Wisent, 320-036-CL) supplemented with 10% of fetal bovine serum. All cells were cultured at 37 °C under a humidified 95–5% (v/v) mixture of air and CO₂. The TNBC cells secretome was generated upon a 48 h serum deprivation of a ~70% confluent MDA-MB-231 culture. Next, the conditioned media (CM) was harvested and centrifuged at 1500× g for 20 min to eliminate cell debris. CM was aliquoted and kept at −20 °C. To evaluate the induction of the CAA phenotype, ADMSC were cultured with the TNBC cells secretome in the presence or absence of 10 μM EGCG for 24 h. Then, cells were collected for total RNA extraction, protein isolation, or cell migration studies.