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Anti cd8β

Manufactured by BioXCell

Anti-CD8β is a monoclonal antibody that specifically binds to the CD8β subunit of the CD8 co-receptor found on the surface of cytotoxic T cells. It is a laboratory tool used for the identification and study of CD8+ T cells.

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3 protocols using anti cd8β

1

Cell Depletion for Immunological Studies

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For cell depletion experiments using neutralizing antibodies, mice were injected with 200 μg/mouse of either anti-CD8β (Clone 53–5.8, BioXcell), anti-CD4 (Clone GK1.5, BioXcell), anti-NK1.1 (Clone PK136, BioXcell) and the respective isotype controls (BioXcell). For cell depletion experiments in conditional knockout mice (Ccr2DTR), 10 ng/g body weight of diphteria toxin (DT) were injected intraperitoneally on day −1, day 1 and day 3 relative to time of vaccination.
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2

Antibody-Mediated T Cell Depletion and Treg Abrogation

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The following antibodies were given i.p. at 200µg per injection: anti-CD8α (Clone: 2.43; BioXcell), anti- CD8β (Clone: 53-5.8; BioXcell), anti-CD25 (Clone: PC61; eBioscience), as well as rat IgG2B (Clone: LTF-2; BioXcell) and rat IgG1 (Clone: HRPN; BioXcell) isotype controls. For in vivo TReg cell depletion with Diphteria Toxin (DT; Sigma-Aldrich), Foxp3DTR mice were given 50µg/kg of DT i.p. every other day as previously described (28 (link),29 (link)).
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3

Cell Depletion for Immunological Studies

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For cell depletion experiments using neutralizing antibodies, mice were injected with 200 μg/mouse of either anti-CD8β (Clone 53–5.8, BioXcell), anti-CD4 (Clone GK1.5, BioXcell), anti-NK1.1 (Clone PK136, BioXcell) and the respective isotype controls (BioXcell). For cell depletion experiments in conditional knockout mice (Ccr2DTR), 10 ng/g body weight of diphteria toxin (DT) were injected intraperitoneally on day −1, day 1 and day 3 relative to time of vaccination.
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