Z serum sep clot activator

Manufactured by Greiner

Sourced in Austria

The Z Serum Sep Clot Activator is a laboratory equipment used for the separation of serum from whole blood samples. It is designed to facilitate the coagulation process and the subsequent separation of the serum component from the cellular fraction of the blood.

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Lab products found in correlation

Ctnt elisa kit, by MyBioSource (2 mentions)

Spelling variants of this product

Vacuette® Z serum Sep Clot Activator (10 citations) Clot activator (9 citations) Serum Clot Activator (6 citations) Z serum clot activator (4 citations) VACUETTE® Z Serum Sep Clot Activator tubes (3 citations) Z Serum Sep Clot Activator tubes (3 citations)

6 protocols using z serum sep clot activator

Blood Sampling and Serum Storage

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Blood samples taken from the antecubital vein were collected in Vacutainers Tubes (5 mL Z Serum Sep Clot Activator, REF 456234, Greiner Bio‐one). Participants were informed to take water but not food overnight, before blood sampling in the morning. Samples were centrifuged (3000 r.p.m. using centrifuge J6‐MC by Beckman), and the serum was stored at −80 °C. All samples were analysed using the same reagent lot.

Li C., Yu K., Shyh‐Chang N., Li G., Jiang L., Yu S., Xu L., Liu R., Guo Z., Xie H., Li R., Ying J., Li K, & Li D. (2019). Circulating factors associated with sarcopenia during ageing and after intensive lifestyle intervention. Journal of Cachexia, Sarcopenia and Muscle, 10(3), 586-600.

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Plasma and Serum Extraction Protocol

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A total of 4 ml venous blood was withdrawn from each subject; 2 ml were collected in EDTA K3 vacutainers for the lamin B1 assay and centrifuged at 1500
g for 10 minutes. Plasma was collected, aliquoted and stored at −70°C. The remaining 2 ml were collected in sterile vacutainers with a Z Serum Sep Clot Activator (Greiner Bio-One). Afterwards, blood was centrifuged for 10 min at 1000
g, the serum was used for immediate analysis of AFP.

Abdelghany A.M., Rezk N.S., Osman M.M., Hamid A.I., Al-Breedy A.M, & Abdelsattar H.A. (2018). Using Lamin B1 mRNA for the early diagnosis of hepatocellular carcinoma: a cross-sectional diagnostic accuracy study. F1000Research, 7, 1339.

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Longitudinal Liquid Biopsy Sampling

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Peripheral blood samples were obtained throughout patient follow-up; before starting any type of systemic treatment, after radiological reassessment (the radiological assessment was performed after neoadjuvant treatment, after 2–3 months of this neoadjuvant treatment), 48 h after surgical procedure, and a year after surgery or at radiological progression of the disease (whichever happened earlier). Blood was collected in EDTA containing tubes (Greiner Bio-One, Kremsmünster, Austria) for plasma and immune cell population isolation, and in clot accelerator tubes Z Serum Sep Clot Activator (Greiner Bio-One, Kremsmünster, Austria) for serum isolation. All blood samples were processed freshly. Tumor samples were obtained only from those patients that could be resected surgically.

González-Borja I., Viúdez A., Alors-Pérez E., Goñi S., Amat I., Ghanem I., Pazo-Cid R., Feliu J., Alonso L., López C., Arrazubi V., Gallego J., Pérez-Sanz J., Hernández-García I., Vera R., Castaño J.P, & Fernández-Irigoyen J. (2022). Cytokines and Lymphoid Populations as Potential Biomarkers in Locally and Borderline Pancreatic Adenocarcinoma. Cancers, 14(23), 5993.

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Serum Sampling for Cardiac Biomarkers in Rat MI

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To obtain serum samples from rats, blood was drawn from the right atrium at 4 h after ligation of the LAD coronary artery with a 22-gauge needle. One millilitre of collected blood was transferred into a Z Serum Sep Clot Activator (Greiner Bioone, Kremsmunster, Austria), followed by centrifugation for 15 min at 3,000 rpm. The cTnT concentrations were determined using an cTnT ELISA kit (MBS2024997, MyBioSource, San Diego, CA, USA) and and HMGB1 concentrations were determined using the Rat HMGB1 ELISA kit (Solarbio, Beijing, China).

Beom J.H., Kim J.H., Seo J., Lee J.H., Chung Y.E., Chung H.S., Chung S.P., Kim C.H, & You J.S. (2021). Targeted temperature management at 33°C or 36℃ induces equivalent myocardial protection by inhibiting HMGB1 release in myocardial ischemia/reperfusion injury. PLoS ONE, 16(1), e0246066.

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Rat Serum Biomarker Quantification

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To obtain rat serum, we punctured the right atrium and obtained blood 4 h after LAD ligation with a 22-gauge needle. We transferred 1 mL of the collected blood into a Z Serum Sep Clot Activator (Greiner Bioone, Kremsmunster, Austria), followed by centrifugation at 3000 rpm for 15 min. We determined the concentrations of cTnT and HMGB1 using a cTnT ELISA kit (MBS2024997, MyBioSource, San Diego, CA, USA) and a HMGB1 ELISA kit (Solarbio, Beijing, China) for rats.

Myung J., Beom J.H., Kim J.H., Woo J.S., Park I., Chung S.P., Chung Y.E, & You J.S. (2022). Recombinant Klotho Protein Ameliorates Myocardial Ischemia/Reperfusion Injury by Attenuating Sterile Inflammation. Biomedicines, 10(4), 894.

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Hepatitis C Serum Sampling

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Serum samples were obtained retrospectively from twenty-six chronic Hepatitis C (HCV) infected patients during participation in a natural history study of patients with liver diseases at the National Institutes of Health Clinical Center (NCT00001971). Patients were evaluated in the outpatient clinic with routine laboratory assessment for HCV associated liver disease and to rule out other forms of chronic liver disease. All patients signed consent for collection, storage, and use of their serum for future research. Samples were collected from HCV patients at two time-points: the earliest available sample from each patient was designated as time-point-A and the most recent sample prior to any therapy as time-point-B. Mean duration between earliest to latest time-point for all patients was approx. 6.8 years. Serum samples from anonymized healthy donors were obtained from NIH Clinical Center blood bank. Blood from an antecubital vein was drawn into a 3.5 ml Z Serum Sep. Clot Activator (Ref 454067P, Greiner Bio-One GmbH, Kremsmunster, Austria). All control and HCV serum samples were processed by centrifugation at 1000 rpm for 10 minutes within 4 hours of being drawn and subsequently stored at −80°C until analysis.

Ali R.O., Moon M.S., Townsend E.C., Hill K., Zhang G.Y., Bradshaw A., Guan H., Hamilton D., Kleiner D.E., Auh S., Koh C, & Heller T. (2019). Exploring the link between platelet numbers and vascular homeostasis across early and late stages of fibrosis in hepatitis C. Digestive diseases and sciences, 65(2), 524-533.

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