Expression of melanoma stem cell markers, were estimated by labeling G-361 cells with anti CD133 antibody (BD Biosciences, USA) or anti ABCB5 antibody (Abcam, Korea). All samples were incubated for 20 min at 4 °C, washed twice with PBS, and immediately analyzed using a BD FACSVerse cytometer and the FACS suite software.
Anti cd133 antibody
Manufactured by BD
Sourced in United States
The Anti CD133 antibody is a laboratory tool used to detect and identify the CD133 protein, which is a cell surface marker. It can be used in various applications related to the study and analysis of CD133-expressing cells, such as in flow cytometry, immunohistochemistry, and Western blotting. The antibody provides a way to observe and quantify the presence of CD133 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Facsverse cytometer, by BD (1 mentions)
Anti h3, by Cell Signaling Technology (1 mentions)
Anti β catenin, by BD (1 mentions)
Anti β actin, by Cell Signaling Technology (1 mentions)
Anti h3k4me3, by Cell Signaling Technology (1 mentions)
Anti eea1, by Cell Signaling Technology (1 mentions)
Anti pgk1, by Cell Signaling Technology (1 mentions)
Anti tom70, by Proteintech (1 mentions)
Anti tom40, by Proteintech (1 mentions)
Anti c myc, by Proteintech (1 mentions)
2 protocols using anti cd133 antibody
1
Melanoma Stem Cell Marker Analysis
2
Antibody and Detection Reagent Purchasing
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Anti-β-Catenin (catalog no. 610153) and anti-CD133 antibody (catalog no. 566598) was purchased from BD Bioscience. Anti-PGK1 (catalog no. 68540 S), anti-EEA1 (catalog no. 3288 S), anti-β-actin (catalog no. 4970), anti-H3 (catalog no. 4499) and anti-H3K4me3 (catalog no. 9751 S) antibodies were from Cell Signaling Technology. Anti-ZIC2 (catalog no. ARP35821_P050) antibody was purchased from Aviva Systems Biology. Anti-TOM40 (catalog no. 18409-1-AP), anti-TOM70 (catalog no. 14528-1-AP), anti-c-MYC (catalog no. 10828-1-AP) and anti-AXIN2 (catalog no. 20540-1-AP) antibodies were from Proteintech Group, Inc. Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 594 antibody (catalog no. A-11005), Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (catalog no. A-11008), Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647 (catalog no. A-21244) were purchased from Invitrogen. HRP-conjugated Affinipure Goat Anti-Mouse IgG(H + L) antibody (catalog no. SA00001-2) and HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H + L) antibody (catalog no. SA00001-2) were purchased from Proteintech Group, Inc. Polymer HRP and AP detection kits were from Beyotime Biotechnology. Biotin labeled RNA mix (catalog no. 11685597910) was from Roche.
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