Immunohistochemistry (IHC) was carried out using three primary antibodies targeting PV L1 antigen (clone BPV1/1H8+CAMVIR; Abcam, Cambridge, MA, U.S.A), retinoblastoma protein (pRb) (clone G3-245; BD Pharmingen, Tokyo, Japan), and p53 (clone FL-393; Santa Cruz Biotechnology, Santa Cruz, CA, U.S.A.). The numbers of immunopositive tumor cells were counted in 5 high-power fields (x400).
Prb clone g3 245
Manufactured by BD
Sourced in Japan
The PRb) (clone G3-245) is a laboratory equipment product. It serves a core function, but a detailed description while maintaining an unbiased and factual approach is not available.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using prb clone g3 245
1
Immunohistochemical Analysis of Tumor Markers
2
Western Blot Antibody Detection Protocol
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SDS-PAGE and western blot were performed following standard protocols as described [50 (link)]. The following antibodies were applied for protein detection: E2F4 (C-20), p130 (C-20), E2F1 (C-20), E2F3 (C-18) (Santa Cruz Biotechnology), LIN54 A303-799A (Bethyl Laboratories), LIN9 ab62329 (Abcam), and pRB clone G3-245 (BD Pharmingen). The polyclonal LIN37 antibody (LIN37-T3) was produced by immunizing rabbits with the peptide CRFPHQRRKKRREMDDGLAE followed by affinity purification of specific antibodies (Pineda Antikörper-Service, Berlin, Germany). The monoclonal B-Myb LX015.1 antibody (hybridoma media 1:5) was a kind gift from Roger Watson [51 (link)].
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