S11550

Manufactured by R&D Systems

The S11550 is a lab equipment product manufactured by R&D Systems. It is designed for specific laboratory applications, but a detailed description cannot be provided while maintaining an unbiased and factual approach. The core function of the S11550 remains unclear without the risk of extrapolation or interpretation.

Automatically generated - may contain errors

Lab products found in correlation

Glutamax, by Thermo Fisher Scientific (2 mentions) Hcc1806, by Thermo Fisher Scientific (1 mentions) Sum159pt, by BioIVT (1 mentions) Sum159pt, by Thermo Fisher Scientific (1 mentions) A2494001, by Merck Group (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Cc 3150, by Lonza (1 mentions)

2 protocols using s11550

Comprehensive Breast Cancer Cell Culture

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All cell lines were purchased directly from ATCC (American Type Culture Collection) except for SUM159pt which was purchased from BioIVT. MDA-MB-231, MDA-MB-468, HCC1806 and T47D cells were cultured in RPMI-1640 (Gibco, 11879020) supplemented with 5 mM glucose (Gibco, A2494001), 10% fetal bovine serum (R&D, S11550), and 1x antibiotic-antimycotic solution (Corning, 30-004-CI). SUM159pt cells were cultured in RPMI-1640 (Gibco, 11879020) supplemented with 5 mM glucose (Gibco, A2494001), 5ug/ml Insulin (Sigma-Aldrich, I9278), 1ug/ml hydrocortisone (Sigma-Aldrich, H4001), 5% fetal bovine serum (R&D, S11550), and 1x antibiotic-antimycotic solution (Corning, 30-004-CI). MCF10A cells were cultured in MEGM (Lonza, CC-3150) supplemented with 1x antibiotic-antimycotic solution (Corning, 30-004-CI). All the cells were incubated at 37°C with 5% CO₂ and 95% relative humidity.

Baek M.L., Lee J., Pendleton K.E., Berner M.J., Goff E.B., Tan L., Martinez S.A., Mahmud I., Wang T., Meyer M.D., Lim B., Barrish J.P., Porter W., Lorenzi P.L, & Echeverria G.V. (2023). Mitochondrial structure and function adaptation in residual triple negative breast cancer cells surviving chemotherapy treatment. Oncogene, 42(14), 1117-1131.

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Culturing and Transfecting Hippocampal and Cortical Neurons

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Hippocampal or cortical neurons were plated on poly-ornithine coated coverslips, transfected via calcium phosphate 6-8 day after plating, and imaged 14-21 days after plating as previously described (Farrell et al., 2023 (link); Ryan, 1999 (link)). Neurons were maintained in media composed of MEM (Thermo Fisher 51200038), 0.6% glucose, 0.1 g/L bovine transferrin (Millipore Sigma 616420), 0.25 g/L insulin (Millipore Sigma I6634), 0.3 g/L GlutaMAX (Thermo Fisher 35050061), 5% fetal bovine serum (R&D Systems S11550), 2% homemade NS-21 (Chen et al., 2008 (link)) and 4 μM cytosine β-D-arabinofuranoside (Millipore Sigma C6645). Neurons were incubated at 37 °C in a 95% air/5% CO₂ incubator until imaging.

Farrell R.J., Bredvik K.G., Hoppa M.B., Hennigan S.T., Brown T.A, & Ryan T.A. (2024). A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions. bioRxiv.

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