Male C57BL/6J (BL6) mice were obtained from Jackson Laboratory (Bar Harbor, ME), housed in a temperature and humidity-controlled micro-isolation facility, fed regular chow and water ad libitum, and studied at ~14 weeks of age (Table 1 ). The study protocols were approved by the Johns Hopkins Animal Care and Use Committee (JHACUC), and all animal experiments were conducted in accordance with the JHACUC guidelines. Age and weight are reported for male mice from studies in previous experiments on mouse upper airway control (17 (link), 18 (link)).
C57bl 6j bl6 mice
Manufactured by Jackson ImmunoResearch
Sourced in Montenegro, United States
C57BL/6J (BL6) mice are an inbred mouse strain commonly used in biomedical research. They are widely utilized as a reference strain and provide a standardized genetic background for various experiments.
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8 protocols using c57bl 6j bl6 mice
1
Mouse Upper Airway Control Protocol
2
Conditional Cox-2 Knockout Mice Model
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The procedures for all animal experiments were approved by the UCLA Animal Research Committee, and all experiments were carried out in accordance with the approved guidelines. Cox2fl/fl;LysMCre+/– (Cox2 MKO) mice, Cox2fl/fl; LysMCre–/– (FLOX), and Cox2luc/luc (Cox2 KO) mice were bred and genotyped as previously described.5 ,74 (link) C57Bl/6J (BL6) mice were from the Jackson Laboratory (Bar Harbor, ME). All mice used in experiments were approximately 4–6 months old. Equal numbers of male and female mice were used when possible. For studies involving CCHF diet (TD.88051; Envigo, Indianapolis, IN), mice were fed for either 2 weeks or 10 weeks as indicated.
3
Anterior Cruciate Ligament Injury Model
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Ten-week-old male C57Bl/6J (BL6) mice (purchased from Jackson Laboratory Bar Harbor, ME, USA; Stock No: 000664) were subjected to anterior cruciate ligament injury using a single non-invasive tibial compressive overload, as previously described [12 (link),13 (link),18 (link),19 (link)]. Tibial compression was applied at 1 mm/s, and loading was manually stopped immediately after ACL injury.
4
In Vivo Bone Imaging in Mice
5
Age-related Study in C57BL/6J Mice
6
Aging Effects on Interferon-γ Knockout Mice
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Male and female wild-type (WT) C57BL/6J (BL6) mice were purchased from Jackson Laboratory (Bar Harbor, ME, USA). Genetic interferon-γ knock out mice on the BL6 background (B6.129S7-Ifngtm1Ts/J, [IFNγKO]) were purchased from Jackson. Mice were purchased at ages 6–8 weeks and aged at our institutional facility, maintained under specific pathogen-free conditions, and given food and water ad libitum throughout their lives. Age- and sex-matched mice that were young (2– 4 months) or aged (19–30 months) were used for all experiments.
7
Evaluating Cisplatin Efficacy in Murine Ovarian Cancer
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Female C57BL/6J (BL/6) mice were purchased from Jackson Laboratories (Bar Harbor, ME) at 6 weeks of age. Female NOD.Cg-Prkdc < scid > Il2rg < tm1Wjl > SzJ (NSG) mice were purchased from the Cleveland Clinic BRU at 6 weeks of age.
Experimental animals were housed and handled in accordance with Cleveland Clinic Lerner Research Institute IACUC guidelines. Mice (BL/6 or NSG) were given either control or antibiotic (0.5 g/L vancomycin, 1 g/L neomycin sulfate, 1 g/L metronidazole, 1 g/L ampicillin) (ABX) (Fisher Scienti c) containing water for 2 weeks prior to IP injection of ID8-LUC (5x10 6 ) ID8-VEGF (5x10 6 ) or OV81 (5x10 6 ) cells. The ABX containing water has been previously shown to be su cient to deplete all detectable commensal bacteria (27, 38) . Mice remained on either control or ABX containing water for the duration of the study. At 10 weeks of age, mice were treated IP with either cisplatin (0.5mg/kg, Spectrum Chemical or vehicle (phosphate buffered saline, PBS) weekly until human endpoint of total tumor burden exceeding 150mm 3 or debilitating ascites development.
Experimental animals were housed and handled in accordance with Cleveland Clinic Lerner Research Institute IACUC guidelines. Mice (BL/6 or NSG) were given either control or antibiotic (0.5 g/L vancomycin, 1 g/L neomycin sulfate, 1 g/L metronidazole, 1 g/L ampicillin) (ABX) (Fisher Scienti c) containing water for 2 weeks prior to IP injection of ID8-LUC (5x10 6 ) ID8-VEGF (5x10 6 ) or OV81 (5x10 6 ) cells. The ABX containing water has been previously shown to be su cient to deplete all detectable commensal bacteria (27, 38) . Mice remained on either control or ABX containing water for the duration of the study. At 10 weeks of age, mice were treated IP with either cisplatin (0.5mg/kg, Spectrum Chemical or vehicle (phosphate buffered saline, PBS) weekly until human endpoint of total tumor burden exceeding 150mm 3 or debilitating ascites development.
8
Tumor Modeling in Mice with Antibiotics
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Female C57BL/6J (BL/6) mice were purchased from Jackson Laboratories (Bar Harbor, ME) at 6 weeks of age. Female NOD.Cg-PrkdcIl2rgSzJ (NSG) mice were purchased from the Cleveland Clinic BRU and Gnotobiotic Core Facility respectively at 6 weeks of age. Experimental animals were housed and handled in accordance with Cleveland Clinic Lerner Research Institute IACUC guidelines. Mice (BL/6 or NSG) were given either control or antibiotic (0.5 g/L vancomycin, 1 g/L neomycin sulfate, 1 g/L metronidazole, 1 g/L ampicillin) (ABX) (Fisher Scienti c) containing water for 2 weeks prior to intraperitoneal (IP) injection of ID8-LUC (5x10 6 ) ID8-VEGF (5x10 6 ) or OV81 (1x10 6 ) cells. The ABX containing water has been previously shown to be su cient to deplete all detectable commensal bacteria (32, 33) . Mice remained on either control or ABX containing water for the duration of the study. At 10 weeks of age, mice were treated IP with either cisplatin (5mg/kg, Spectrum Chemical or vehicle (PBS) weekly until human endpoint of total tumor burden exceeding 150mm 3 or debilitating ascites development.
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