For culture-inoculated fly food analysis, Lp and At were grown overnight in MRS media and adjusted to OD600=0.5. 150 μl of Lp, At, or a 1:1 mixture of Lp and At was applied to the surface of fly food and allowed to soak in for 3 days at 25°C. For gnotobiotic fly-inoculated fly food analysis, 40 axenic/gnotobiotic male flies were sorted aseptically, transferred to sterile fly food, and incubated for 3 days at 25°C. Fly food was removed from the vial and weighed, vortexed vigorously with deionized water, and the pH was measured using an Orion Star pH meter (ThermoFisher).
Orion star ph meter
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
The Orion Star pH meter is a digital pH measuring instrument used for precise pH determination in various applications. It offers accurate and reliable pH measurement capabilities.
Automatically generated - may contain errors
Lab products found in correlation
Pb clo4 2 3h2o, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
N acetyl l cysteine, by Merck Group (1 mentions)
Agilent 7890b gas chromatograph, by Agilent Technologies (1 mentions)
Ultrapure water, by Thermo Fisher Scientific (1 mentions)
Methacrylic acid, by Merck Group (1 mentions)
Type a porcine skin gelatin, by Merck Group (1 mentions)
Sephadex g 15, by GE Healthcare (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
8 protocols using orion star ph meter
1
Inoculated Fly Food Analysis
2
Synthesis of Lead-based Compounds
3
Comprehensive Wastewater Characterization
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The total solids (TS), the total volatile solids (TVS), the suspended solids (SS), and the volatile suspended solids (VSS) content were characterized according to standard methods (APHA, 2005). Soluble and total chemical oxygen demand (sCOD and tCOD) were also measured according to standard methods (APHA, 2005). The pH was measured using a Thermo Scientific Orion Star pH meter. Volatile fatty acids (VFAs), including acetic acid, propionic acid, butyric acid, valeric acid, and caproic acid were quantified via GC-FID using an Agilent 7890B gas chromatograph (Santa Clara, CA, USA) equipped with a Nukol capillary column (30 m × 0.25 mm × 0.25 µm). Cations (Na+, NH4+, K+) were measured by injecting a sample volume of 20 µL into a high-performance liquid chromatograph (HPLC) (Waters Chromatography, Milford, MA, USA) equipped with a Hamilton PRP-X200 cation resin-based column (250 × 41 mm O.D.), a conductivity detector (Waters Millipore model 432), and an Empower2 data station.
Measurements of H2, N2, O2, CO, CH4, and CO2 were made on an Agilent 7820 gas chromatograph (Wilmington, DE, USA) coupled to a thermal conductivity detector (TCD). The gas sample was injected on a 2 m × 2 mm I.D. ShinCarbon ST packed column from RESTEK. Argon was used as carrier gas at a flow rate of 10 mL min−1.
Measurements of H2, N2, O2, CO, CH4, and CO2 were made on an Agilent 7820 gas chromatograph (Wilmington, DE, USA) coupled to a thermal conductivity detector (TCD). The gas sample was injected on a 2 m × 2 mm I.D. ShinCarbon ST packed column from RESTEK. Argon was used as carrier gas at a flow rate of 10 mL min−1.
4
Quantifying Glass Disc Degradation
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The degradation of the glass discs (n = 3) was quantified after one, four, seven and 14 days storage in 25 ml deionised water (pH 7.0 ± 0.1) by measuring the mass change of the specimens, the ion release and the pH change of the solution. At each time point, specimen extracts were collected for pH change (Orion Star pH Meter, Thermo Scientific, UK) and ion chromatography measurements. Glass samples were dried and weighed at each time point for determination of mass change, before being transferred to fresh deionised water. Mass change was calculated according to equation (2)
where Δm, m0, mt and A are the change in mass (g), initial mass, mass at specific time point and surface area of the specimen (cm2), respectively.
where Δm, m0, mt and A are the change in mass (g), initial mass, mass at specific time point and surface area of the specimen (cm2), respectively.
5
Poultry Carcass Characteristics Assessment
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At the end of the experiment, birds from each group were weighed individually and sacrificed by cervical dislocation and bleeding via jugular vein. Carcasses were weighed prior to being skinned and deboned. Breast muscle, thigh muscle and abdominal fat were removed, weighed and expressed as a percentage of body weight. Breast muscle from the right side was used to determine the carcass characteristics. The breast meat lightness (L*), redness (a*) and yellowness (b*) values were measured with an SP64 color-difference meter (X-Rite, Grand Rapids, MI, USA), and pH values were detected at 45 min postmortem using an Orion Star pH meter (Thermo Fisher Scientific, Waltham, MA, USA). Drip loss was measured using approximately 5 g of meat sample according to the plastic bag method described by Honikel (1998) [20 (link)], and shear force values of breast muscle were measured using an RH-N50 meat tenderness tester (Mingao, Nanjing, China).
6
Synthesis and Purification of Methacrylated Gelatin
7
Methionine Purification and Characterization
8
Disc Storage pH Evaluation
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Three discs from each composition were stored in 1 ml Dulbecco Modified Eagles Medium (DMEM) (Gibco®, Life Technologies Ltd., Paisley, UK), pH 8.4± 0.1, for 1, 4 and 7 days. At each time point, the pH of the specimen extract was measured using pH detector; Orion Star pH Meter (Thermo Scientific, UK). The culture media was changed every 3 days to mimic the environment of the cell culture study. Samples were stored in a 37°C/5% CO2 incubator in between time points. The culture media without discs was used as control.
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