Statistical analysis was performed by using GraphPad
Prism Program 7.0 software (RRID:SCR_000306, GraphPad, San Diego, CA, USA). We used one-way ANOVA followed by Dunnett’s post hoc test when comparing more than two groups of data and one-way ANOVA, nonparametric Kruskal–Wallis test, followed by Dunnett’s post hoc test when comparing multiple independent groups.
P values of 0.05 were considered to be statistically significant. Post hoc tests were run only if
F achieved
P < 0.05, and there was no significant in homogeneity of variance. No sample calculation was performed. We did not conduct any data test for outliers, and thus, no single data points were excluded. All the data were expressed as the mean ± SEM, at least three individual experiments (
n = number of independent cell culture preparations). The
n values are provided in the figure legends. Error bars were omitted when they fell within the dimensions of the symbols.
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