Nih 31 modified open formula

Eight-week old, healthy, male Wistar-Han rats (Charles River Laboratories, Raleigh, NC) were randomly assigned to receive intraperitoneal (IP) injections of endotoxin-free saline (vehicle) or 200µg/kg zoledronic acid (ZA) (LKT Laboratories, St Paul, MN) twice weekly, in morning hours (219–261g, 236g average). Vehicle (Veh) or ZA treatment was continued throughout the duration of the experiments. Throughout the experiment, rats were housed in pathogen-free conditions (2 per cage) with a 12-hour light/dark cycle, fed a standard diet (NIH-31 Modified Open Formula, ENVIGO, Madison, WI) and given water ad libitum. Rats with retained root fragments or fractured cortical plates were excluded from analysis. All applicable institutional and/or national guidelines for the care and use of animals were followed.

This study was approved by the UCLA Chancellor’s Animal Research Committee (ARC). Four-week old male C57BL/6 mice (Charles River Laboratories, Raleigh, NC) were utilized for these experiments, following ARRIVE guidelines. A standard diet (NIH-31 Modified Open Formula, ENVIGO, Madison, WI, USA) and water were provided ad libitum. Animals received intraperitoneal (IP) injections of Veh (endotoxin-free saline), or 200 μg/kg Zoledronic Acid (LKT Laboratories, St Paul, MN) two times weekly through the duration of the experiment.

This study was approved by the UCLA Chancellor’s Animal Research Committee (ARC). Animals were housed following the ARC guidelines. Rats (1 per cage) were boarded in pathogen-free conditions with a 12-hour light/dark cycle. A standard diet (NIH-31 Modified Open Formula, ENVIGO, Madison, WI, USA) and water were provided ad libitum. A randomized, controlled, animal model design was utilized for this prospective study, following all the recommendations of the Animal Research: Reporting In Vivo Experiments (ARRIVE) guidelines.