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Anti pe biotin antibody clone pe001

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The Anti-PE-biotin antibody (clone PE001) is a laboratory reagent used for the detection and enrichment of cells or molecules labeled with phycoerythrin (PE). This antibody specifically binds to the PE molecule, allowing for its identification and isolation in various experimental procedures.

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Lab products found in correlation

Streptavidin pe, by BioLegend (2 mentions) Primary osmr pe antibody clone an v2, by BioLegend (2 mentions)

Spelling variants of this product

PE-anti-biotin antibody (2 citations) PE-biotin (2 citations)

2 protocols using anti pe biotin antibody clone pe001

1

Staining OSMR in Human Cells for Flow Cytometry

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To stain OSMR in human samples for flow cytometry analysis, cells were labeled with primary OSMR-PE antibody (clone AN-V2, 2 µg/ml) followed by three rounds of amplification with anti-PE-biotin antibody (clone PE001, 2.5 µg/ml (Biolegend, UK)), and streptavidin-PE (0.4 µg/ml (Biolegend, UK)). A separate cell sample was labeled with isotype control antibody (mouse IgG1-PE) and similarly amplified to control for background staining. Specificity of the anti-OSMR antibody was confirmed by siRNA knockdown of OSMR expression in a prior publication65 (link).
West N.R., Hegazy A.N., Owens B.M., Bullers S.J., Linggi B., Buonocore S., Coccia M., Görtz D., This S., Stockenhuber K., Pott J., Friedrich M., Ryzhakov G., Baribaud F., Brodmerkel C., Cieluch C., Rahman N., Müller-Newen G., Owens R.J., Kühl A.A., Maloy K.J., Plevy S.E., Keshav S., Travis S.P, & Powrie F. (2017). Oncostatin M drives intestinal inflammation in mice and its abundance predicts response to tumor necrosis factor-neutralizing therapy in patients with inflammatory bowel disease. Nature medicine, 23(5), 579-589.
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2

Staining OSMR in Human Cells for Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
To stain OSMR in human samples for flow cytometry analysis, cells were labeled with primary OSMR-PE antibody (clone AN-V2, 2 µg/ml) followed by three rounds of amplification with anti-PE-biotin antibody (clone PE001, 2.5 µg/ml (Biolegend, UK)), and streptavidin-PE (0.4 µg/ml (Biolegend, UK)). A separate cell sample was labeled with isotype control antibody (mouse IgG1-PE) and similarly amplified to control for background staining. Specificity of the anti-OSMR antibody was confirmed by siRNA knockdown of OSMR expression in a prior publication65 (link).
West N.R., Hegazy A.N., Owens B.M., Bullers S.J., Linggi B., Buonocore S., Coccia M., Görtz D., This S., Stockenhuber K., Pott J., Friedrich M., Ryzhakov G., Baribaud F., Brodmerkel C., Cieluch C., Rahman N., Müller-Newen G., Owens R.J., Kühl A.A., Maloy K.J., Plevy S.E., Keshav S., Travis S.P, & Powrie F. (2017). Oncostatin M drives intestinal inflammation in mice and its abundance predicts response to tumor necrosis factor-neutralizing therapy in patients with inflammatory bowel disease. Nature medicine, 23(5), 579-589.
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