Mlecs

Manufactured by Cell Biologics

MLECs (Mouse Lung Endothelial Cells) are primary cells derived from the lungs of mice. They are designed to maintain the characteristics and functions of endothelial cells found in the mouse lung vasculature. MLECs provide a physiologically relevant in vitro model for studying endothelial cell biology and vascular-related research applications.

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Lab products found in correlation

Gelatin based coating solution, by Cell Biologics (1 mentions) Cell culture medium, by Cell Biologics (1 mentions) Pericyte medium, by ScienCell (1 mentions) Bend 3, by American Type Culture Collection (1 mentions) B16f10 cells, by American Type Culture Collection (1 mentions)

3 protocols using mlecs

Primary Mouse Lung Endothelial Cells

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Primary mouse lung microvascular endothelial cells (MLECs) and complete mouse endothelium cell medium were both purchased from Cell Biologics, (Chicago, IL). Cells were maintained in 10-cm plastic dishes pre-coated with Gelatin-Based Coating Solution (Cell Biologics, Chicago, IL). For some experiments, MLECs were cultured with serum from lean or obese mice (10 μl serum/ml complete mouse endothelium cell medium containing 1% FBS). At pre-specified time points, cell lysates were collected for later analysis. All in vitro studies were performed using cells from passage 3–4.

Shah D., Romero F., Duong M., Wang N., Paudyal B., Suratt B.T., Kallen C.B., Sun J., Zhu Y., Walsh K, & Summer R. (2015). Obesity-induced adipokine imbalance impairs mouse pulmonary vascular endothelial function and primes the lung for injury. Scientific Reports, 5, 11362.

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Hyperoxia Exposure of Lung Cells

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MLECs were purchased from Cell Biologics (Chicago, IL) and maintained in cell culture medium (Cell Biologics, Chicago, IL) as previously described [24 ]. Exposure to hyperoxia was achieved by leaving the plates inside a tightly sealed modular chamber (Stem Cell Technologies, Vancouver, Canada) filled with 100% oxygen for 16 h.

Gilfillan M., Das P., Shah D., Alam M.A, & Bhandari V. (2020). Inhibition of microRNA-451 is associated with increased expression of Macrophage Migration Inhibitory Factor and mitgation of the cardio-pulmonary phenotype in a murine model of Bronchopulmonary Dysplasia. Respiratory Research, 21, 92.

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Culturing and Transducing Cell Lines for Cancer Research

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LLC, bEnd3 and B16F10 cells (ATCC) were maintained according to ATCC standard culture instructions. WT31 cells were kindly provided by C. Géraud, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany. WT31 cells were cultured in RPMI-1640 media supplemented with 10% fetal calf serum (FCS) and 1% penicillin-streptomycin. Primary mouse lung microvascular ECs (mLECs) were purchased from Cell Biologics and were cultured in the manufacturer’s recommended media. Human lung pericytes (LPs) were kindly provided by B. Peault (University of Edinburgh, UK) and cultured in pericyte medium (ScienCell, #1201) supplemented with 2% FCS, 1% of the corresponding pericyte growth supplement and 1% penicillin-streptomycin. LLC cells were transduced with lentivirus to overexpress either Lrg1 or control vector pLenti. All cells were cultured at 37°C and 5% CO₂ and routinely tested for mycoplasma by polymerase chain reaction (PCR).

Singhal M., Gengenbacher N., Pari A.A., Kamiyama M., Hai L., Kuhn B.J., Kallenberg D.M., Kulkarni S.R., Camilli C., Preuß S.F., Leuchs B., Mogler C., Espinet E., Besemfelder E., Heide D., Heikenwalder M., Sprick M.R., Trumpp A., Krijgsveld J., Schlesner M., Hu J., Moss S.E., Greenwood J, & Augustin H.G. (2021). Temporal multi-omics identifies LRG1 as a vascular niche instructor of metastasis. Science translational medicine, 13(609), eabe6805.

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