Rabbit anti snail1

Human recombinant tumor necrosis factor (TNF)-α, interleukin (IL)-1β, epidermal growth factor (EGF), and basic fibroblast growth factor (bFGF) were purchased from Peprotech (Rocky Hill, NJ, USA). Cisplatin, doxorubicin, BMS-345541, and anti-Flag M2 antibody were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). The TLR agonists Pam3Cys, polyI:C, LPS, and R848 were purchased from InvivoGen (San Diego, CA, USA) and CpG-ODNs was obtained from Invitrogen (Carlsbad, CA, USA). Antibodies against human and mouse USP4 were purchased from Gentex (Taipei, Taiwan) and Invitrogen, respectively, anti-PD-L1 from eBioscience/Thermo Fisher (Waltham, MA, USA), and rabbit anti-snail1, anti-phospho-RelA (Alexa Fluor 647 conjugate), and rabbit IgG isotype control antibody from Cell Signaling (Beverley, MA, USA). Reagents for MTS and luciferase assays were purchased from Promega (Madison, WI, USA). A human lung cancer tissue qPCR array (TissueScan Lung Cancer Tissue qPCR Panel III) was purchased from OriGene Technologies (Rockville, MD, USA).

The following antibodies were used: mouse anti- FLAG M2 (Sigma-Aldrich), rabbit anti-HA tag (Abcam), rabbit anti-E-cadherin (Cell Signaling Technology), mouse anti-E-cadherin (DAKO), rabbit anti-ZEB1 (Cell Signaling Technology), rabbit anti-Snail1 (Cell Signaling Technology), rabbit anti-Twist1 (BIORAD), mouse anti-β-catenin (Santa Cruz Biotechnology), mouse anti-TCF4 (Santa Cruz Biotechnology), mouse anti-β-catenin (Sigma-Aldrich), rabbit anti-phospho-STAT3 (Abcam), rabbit anti-STAT3 (Abcam), rabbit anti-galectin-3 (Abcam), goat anti-Trop-2 (R&D), and HRP-conjugated mouse anti-FLAG M2 antibodies (Sigma-Aldrich). Rabbit anti-phospho-Trop-2 antibodies were prepared as described previously (23 (link)).