L cysteine methyl ester hydrochloride

Manufactured by Merck Group

Sourced in United States

L-cysteine methyl ester hydrochloride is a chemical compound used as a laboratory reagent. It is a salt formed by the reaction of L-cysteine, an amino acid, with methanol and hydrochloric acid. The compound is commonly used in various chemical and biochemical applications, such as protein synthesis and analysis.

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L-cysteine (737 citations) Cysteine (321 citations) L-cysteine hydrochloride (83 citations) Cysteine hydrochloride (26 citations) L-cysteine methyl ester (3 citations) Hydrochlorides (2 citations)

4 protocols using l cysteine methyl ester hydrochloride

Characterization of Polysaccharide Structures

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d-(+)-Glucose, d-allose, d-galactose, Ag₂CO₃, L-cysteine methyl ester hydrochloride, phenyl isothiocyanate, pyridine, iPrOH, MTBE, porcine pancreatic α-amylase, acarbose, dinitrosalicylic acid, ACN and MeOH HPLC grade, and n-hexane, CHCl₃, MeOH, ACN, (CH₃)₂CO and EtOAc technical grade were purchased from Sigma-Aldrich (St. Louis, MO, USA). Nano-pure water HPLC grade (18.2 MΩcm) was obtained from a NANO pure purification system (Barnstead/Thermolyne, Dubuque, IA, USA). Silica gel 60 F254 TLC plates, HCl and H₃PO₄ were purchased from EMD Millipore, Inc. (Darmstadt, Germany). Silica gel 60 Å 40-63 µm and AcOH glacial were purchased from VWR International LLC (West Chester, PA, Switzerland), while RP-C₁₈ Cosmosil 140 and formic acid were purchased from Nacalai Tesque, Inc., (Kyoto, Japan) and Alfa Aesar (Ward Hill, MA, USA), respectively. MeOH and pyridine NMR (perdeuterated) solvents were purchased from Cambridge Isotope Laboratories (Andover, MA, USA).

Perera W.H., Shivanagoudra S.R., Pérez J.L., Kim D.M., Sun Y., K. Jayaprakasha G, & S. Patil B. (2021). Anti-Inflammatory, Antidiabetic Properties and In Silico Modeling of Cucurbitane-Type Triterpene Glycosides from Fruits of an Indian Cultivar of Momordica charantia L.. Molecules, 26(4), 1038.

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Synthesis of Cysteine Derivatives

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l-cysteine methyl ester hydrochloride, o-tolyl isothiocyanate, d-glucose (d-Glc), l-glucose (l-Glc), and DMSO-d₆ were purchased from Sigma-Aldrich Chemical Co. Ltd. (Saint Louis, MO, USA). Methanol (MeOH) was purchased from Yuwang Industrial Co. Ltd. (Yucheng, China). Formic acid (HCOOH) was obtained from Kemiou Chemical Reagent Co. Ltd. (Tianjin, China).

Yang B.J., Chen G.D., Li Y.J., Hu D., Guo L.D., Xiong P, & Gao H. (2016). A New Xanthone Glycoside from the Endolichenic Fungus Sporormiella irregularis. Molecules, 21(6), 764.

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Synthesis and Purification of Thiol-Containing Compounds

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Nicotinic acid, N,N-dimethylformamide, N,N-diisopropylethylamine, L-cysteine methyl ester hydrochloride, hydrazine monohydrate, and iodomethane were purchased from Sigma-Aldrich (St. Louis, MO). Thionyl chloride was obtained from Junsei Chemical Co., Ltd (Tokyo, Japan). Thiopropyl Sepharose™ 6B resins were purchased from GE Healthcare Biosciences (Pittsburgh, PA). C. jejuni NCTC11168 (#700819) and C. concisus RM5485 (#BAA-1457) were from ATCC (Manassas, VA). All other chemicals were of analytical grade.

Jang K.S., Nani R.R., Kalli A., Levin S., Müller A., Hess S., Reisman S.E, & Clemons WM J.r. (2015). A Cationic Cysteine-Hydrazide as an Enrichment Tool for the Mass Spectrometric Characterization of Bacterial Free Oligosaccharides. Analytical and bioanalytical chemistry, 407(20), 6181-6190.

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Carboxylated-CNF Modification and Characterization

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Carboxylated-CNF, provided by RISE Bioeconomy (Stockholm, Sweden), was produced from commercial never-dried bleached sulfite softwood dissolving pulp (lignin content < 1.5%, xylose < 1.7%, mannose < 1.8%, Domsjö Fabriker AB, Örnsköldsvik, Sweden) by 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-mediated oxidation. L-cysteine methyl ester hydrochloride, N-(3-dimethyolaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), collagenase from Clostridium histolyticum, and calcein-AM were obtained from Sigma Aldrich (St. Louis, MO, USA). fetal bovine serum (FBS), penicillinin, streptomycin, 5,5-dithio-bis-(2-nitrobenzoic acid) (DTNB), EnzChek™ Gelatinase/Collagenase Assay Kit, Pierce ^TM Bradford Protein Assay Kit, DMEM:F12 medium, fetal bovine serum, and presto blue cell viability reagent were obtained from ThermoFisher Scientific (Waltham, MA, USA). 1,1-Diphenyl-2-picrylhydrazyl radical (DPPH) was purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

Blasi-Romero A., Palo-Nieto C., Sandström C., Lindh J., Strømme M, & Ferraz N. (2021). In Vitro Investigation of Thiol-Functionalized Cellulose Nanofibrils as a Chronic Wound Environment Modulator. Polymers, 13(2), 249.

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