Turbomass 5

For the identification of the compounds, gas chromatography coupled to mass spectrometry (GC–MS) was performed using a Clarus 500 GC–MS from Perkin-Elmer Inc. apparatus equipped with the same capillary column, carrier, and operating conditions as described above for GC analysis. The ionization source temperature was set at 200 °C and an electron impact mode of 70 eV was employed. MS spectra were obtained by means of total ion scan (TIC) mode (mass range m/z 45–500 uma). The total ion chromatograms and mass spectra were processed with the Turbomass 5.4 software (Perkin-Elmer Inc.). Retention indexes were determined by injection of C₈–C₃₂ n-alkanes standard under the same conditions.
The EO components were identified by comparison of their mass spectra with those of the computer library NIST MS Search 2.0 and available data in the literature [61 ]. Identification of the following compounds was confirmed by comparison of their experimental RI with those of authentic reference standards (Sigma-Aldrich, Darmstadt, Germany): α-pinene, β-pinene, camphene, myrcene, limonene, (Z)-β-ocimene, camphor, borneol, terpinen-4-ol, bornyl acetate, and linalool.

The GC-MS analysis was carried out on Clarus 500 GC-MS (Perkin-Elmer Inc.) apparatus, equipped with the same capillary column and carrier. Its operating conditions are described above for the GC-FID analysis. The ionization source temperature was set at 200°C and the 70 eV electron impact mode was employed. MS spectra were obtained in the full scan mode (mass range m/z 45-500 uma). The total ion chromatograms (TIC) and mass spectra were processed with the Turbomass 5.4 software (Perkin-Elmer Inc.). Retention indices were determined by an injection of the C₈–C₂₅ n-alkanes standard (Supelco, Bellefonte, PE, USA) under the same conditions. The EO components were identified by making a comparison of the calculated retention indices and high probability matches according to a mass spectra computer library search (NIST MS 2.0) and the available data from the literature (Adams, 2007 ). The identification of the following compounds was also confirmed by comparing their experimental linear retention index (LRI) to those of authentic reference standards (Merck KGaA, Darmstadt, Germany): α-pinene, β-pinene, camphene, p-cymene, myrcene, limonene, (Z)-β-ocymene, camphor, terpinolene and terpinen-4-ol.