1,2-DMH, indomethacin, vitamin D and 5-flurouracil (5-FU) were obtained from Sigma Aldrich Co. (Merck KGaA). Certain chemicals used, such as NaCl, EDTA and Tris buffer were purchased from a local commercial source.
5 flurouracil 5 fu
Manufactured by Merck Group
5-Fluorouracil (5-FU) is a synthetic pyrimidine analog used as a laboratory reagent. It functions as a thymidylate synthase inhibitor, which disrupts DNA synthesis. 5-FU is commonly used in biological research and development applications.
Automatically generated - may contain errors
Lab products found in correlation
1 2 dmh, by Merck Group (1 mentions)
Vitamin d, by Merck Group (1 mentions)
Indomethacin, by Merck Group (1 mentions)
Alpha amanitin, by Merck Group (1 mentions)
Radioactively labeled nucleoside triphosphates ntps, by PerkinElmer (1 mentions)
Puromycin, by Merck Group (1 mentions)
Doxorubicin, by Merck Group (1 mentions)
Dmem f12, by Merck Group (1 mentions)
Carboplatin, by Merck Group (1 mentions)
Mitoxantrone, by Merck Group (1 mentions)
Interleukin 3 (il 3), by Thermo Fisher Scientific (1 mentions)
Stem cell factor (scf), by Thermo Fisher Scientific (1 mentions)
293ft cell, by Thermo Fisher Scientific (1 mentions)
Interleukin 6 (il 6), by Thermo Fisher Scientific (1 mentions)
Cyclosporin a csa, by Merck Group (1 mentions)
N acetylcysteine nac, by Merck Group (1 mentions)
6 protocols using 5 flurouracil 5 fu
1
Chemotherapeutic Agents Investigation
2
Synthesis and Assays of NI Compounds
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All NI were synthesized by Gilead Sciences, Inc. Puromycin, 5-flurouracil (5-FU), and alpha-amanitin were purchased from Sigma-Aldrich (St. Louis, MO). All radioactively labeled nucleoside triphosphates (NTPs) were purchased from PerkinElmer (Shelton, CT).
3
Evaluating Drug Resistance in Cancer Cells
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3,000 cells/well in 96-well plates were seeded and cultured in DMEM/F12 (Sigma-Aldrich) supplemented with the necessary growth factors (Sigma-Aldrich). After 7 h of incubation, SP and non-SP cells were treated with 5 µg/ml carboplatin (Sigma-Aldrich), 5 µg/ml 5-flurouracil (5-FU; Sigma-Aldrich) and 5 µg/ml doxorubicin (Sigma-Aldrich). The mean optical density value at 450 nm (OD450) obtained was expressed as a graph. The drug resistance in the experimental groups was calculated using the following formula: Drug resistance rate (%)=(OD450 experimental group/OD450 control group) ×100.
4
Evaluating Chemotherapeutic Agents in Breast Cancer
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MDA-MB-231 and BT-474 cells were treated with 0.25 μM doxorubicin (DOX) or 0.2 μM of mitoxantrone (MXR) or 3 μM of 5-flurouracil (5-FU) (sigma Aldrich) for 4 days in incubator after 4 days cells were harvested for RNA isolation followed by cDNA preparation. For retention studies 1 μM of doxorubicin or 1 μM of mitoxantrone or 0.5 μM of rhodamine 123 was treated for only 1 hour.
5
Generation of Timp1-Overexpressing Murine Bone Marrow Cells
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Mouse Timp1 cDNA was obtained by PCR with Timp1-specific primers and cloned into the retroviral expressing vector MSCV-GFP to generate the MSCV-TIMP1 construct. Retrovirus was produced by calcium phosphate transfection of 293FT cells (Invitrogen) with MSCV-TIMP1 and a helper plasmid pCL-Eco. Retroviral supernatants were collected twice at 36 and 60 h after transfection.
BM cells were harvested at day 5 after mice received i.p. injection of 3 mg/kg 5-flurouracil (5-FU; Sigma-Aldrich) and cultured in DMEM supplemented with 10% FCS (Hyclone), 10 ng/ml IL-3 (PeproTech), 10 ng/ml IL-6 (PeproTech), and 50 ng/ml SCF (PeproTech). Retroviral infection of BM cells was achieved by spin infection using retroviral supernatants according to a standard protocol.
BM cells were harvested at day 5 after mice received i.p. injection of 3 mg/kg 5-flurouracil (5-FU; Sigma-Aldrich) and cultured in DMEM supplemented with 10% FCS (Hyclone), 10 ng/ml IL-3 (PeproTech), 10 ng/ml IL-6 (PeproTech), and 50 ng/ml SCF (PeproTech). Retroviral infection of BM cells was achieved by spin infection using retroviral supernatants according to a standard protocol.
6
Inhibition of Mitochondrial Dysfunction
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NPC-26 was a gift from Dr. Dong's group [33 (link)]. N-acetyl cysteine (NAC) and Mn (III) tetrakis (4-benzoic acid) porphyrin (MnTBAP), a superoxide dismutase mimetic, were provided by Sigma Chemicals (Sigma, St. Louis, MO). mPTP blockers cyclosporin A (CsA) or sanglifehrin A (SfA) and 5-Flurouracil (5-FU) were also obtained from Sigma Chemicals. Antibodies for tubulin and AMPK signaling proteins were purchased from Cell Signaling Tech (Shanghai, China).
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