Pvx cha plates

The strain F. novicida CIP56.12 (Centre de Ressources Biologiques de l'Institut Pasteur, Paris, France) and the ldc mutants were grown on PVX-CHA plates (bioMérieux, Marcy l'Étoile, France) incubated at 37 °C in a 5% CO₂-enriched atmosphere. Liquid cultures were carried out at 25 °C or 37 °C under agitation at 180 rpm in MMH medium, as indicated. For the growth of the complemented strain ΔldcF::ldcF, liquid and solid media were supplemented with kanamycin (10 µg/mL).

F. novicida (U112) CIP56.12 (Centre de Ressources Biologiques de l'Institut Pasteur, Paris, France) and F. philomiragia (CHUGA-FP47) were grown either on solid or in liquid media, as indicated. The solid medium corresponds to pre-made Polyvitex-enriched chocolate agar (PVX-CHA) plates (Ref#43109 BioMérieux, France) incubated at 37°C in a 5% CO₂ incubator with humidified atmosphere and used both to revive glycerol stocks and for CFU counting. Liquid cultures were carried out in Modified Mueller-Hinton broth (MMH) at 37°C under shaking. MMH corresponds to Mueller Hinton Broth (Ref#275730 Grosseron) supplemented with NaCl (Sigma; 5 g/L), BactoProteose peptone (Ref#211693 BD Biosciences; 5 g/L), BactoTryptone (Ref#211705 BD Biosciences 5 g/L) and L-cysteine (Ref#C7477 Sigma; 1 g/L).