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Microfire

Manufactured by Olympus
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The MICROfire is a laboratory equipment product designed for high-quality imaging. It features a compact and versatile design to support various scientific applications.

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Lab products found in correlation

Ix70 inverted microscope, by Olympus (3 mentions) Ix71 microscope, by Olympus (1 mentions)

Close spelling variants of this product

Microfire camera Microfire Digital Camera

4 protocols using microfire

1

Imaging GFP and TurboFP635 in A549 Tumor Cells

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GFP and TurboFP635 expression in A549 cell cultures infected with GLV-1h68 and -2b372 at an MOI of 0.1 was imaged at 24, 48, 72, and 96 hpi using an inverted Olympus IX71 microscope with an Olympus MicroFire digital CCD camera. Whole-field brightness increase by 40% was applied equally across the entire image of all cell-culture GFP images using PowerPoint (Microsoft).
The expression of the GFP protein in GLV-1h68- and -1h376-infected A549 tumors was imaged ex vivo using a Leica M165 FC Fluorescence Classic Stereomicroscope and Leica Application Suite V3.7 from Leica Microsystems.
Tumor-bearing mice infected with GLV-2b372 were anesthetized by isoflurane inhalation and imaged for TurboFP635 expression at 3, 8, and 15 dpi using a Carestream Imager (Bruker). The obtained images and results in relative fluorescence units were processed using Molecular Imaging Software “MI” 7.1 (Bruker) for Windows and Microsoft Excel.
Tsoneva D., Minev B., Frentzen A., Zhang Q., Wege A.K, & Szalay A.A. (2017). Humanized Mice with Subcutaneous Human Solid Tumors for Immune Response Analysis of Vaccinia Virus-Mediated Oncolysis. Molecular Therapy Oncolytics, 5, 41-61.
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2

Microparticle Synthesis via PEG Crosslinking

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Example 4

Heparinated PEG8-VS solutions were combined with PEG8-amine solutions at a 1:1 ratio. The PEG solutions were diluted to 20 mg/mL PEG with PBS and 1.5 M sodium sulfate (in PBS) to a final sodium sulfate concentration of 0.6 M. The PEG8-VS/PEG8-amine solutions were then incubated above the cloud point at 70° C. for 11 min. Suspensions of microparticles were subsequently buffer exchanged into 8 mM sodium phosphate twice to remove the sodium sulfate by: (1) diluting the microparticle solution 3:1 with PBS and titurating, (2) centrifuging at 14,100 g for 2 min, and (3) removing the supernatant. Fluorescent and phase contrast images were captured using a MICROfire (Olympus, Center Valley, Pa.) camera attached to an Olympus IX70 inverted microscope.

US10137082B2. Hydrogel microparticle scaffold with gradients of degradability and methods thereof (2018-11-27). None [US], None [US]. Inventors: Donald L. Elbert [US], Jacob Roam [US].
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3

PEG Microparticle Synthesis and Purification

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Example 4

Heparinated PEG8-VS solutions were combined with PEG8-amine solutions at a 1:1 ratio. The PEG solutions were diluted to 20 mg/mL PEG with PBS and 1.5 M sodium sulfate (in PBS) to a final sodium sulfate concentration of 0.6 M. The PEG8-VS/PEG8-amine solutions were then incubated above the cloud point at 70° C. for 11 min. Suspensions of microparticles were subsequently buffer exchanged into 8 mM sodium phosphate twice to remove the sodium sulfate by: (1) diluting the microparticle solution 3:1 with PBS and titurating, (2) centrifuging at 14,100 g for 2 min, and (3) removing the supernatant. Fluorescent and phase contrast images were captured using a MICROfire (Olympus, Center Valley, Pa.) camera attached to an Olympus IX70 inverted microscope.

US10682309B2. Hydrogel microparticle scaffold with gradients of degradability and methods thereof (2020-06-16). None [US], None [US]. Inventors: Donald L. Elbert [US], Jacob Roam [US].
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4

Synthesis and Characterization of PEG-Based Microspheres

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Heparinated PEG8-VS solutions were combined with PEG8-amine solutions at a 1:1 ratio. The PEG solutions were diluted to 20 mg/mL PEG with PBS and 1.5 M sodium sulfate (in PBS) to a final sodium sulfate concentration of 0.6 M. The PEG8-VS/PEG8-amine solutions were then incubated above the cloud point at 70°C for 11 minutes. Suspensions of microspheres were subsequently buffer exchanged into 8 mM sodium phosphate twice to remove the sodium sulfate by: (1) diluting the microsphere solution 3:1 with PBS and titurating, (2) centrifuging at 14,100g for 2 min, and (3) removing the supernatant. Fluorescent and phase contrast images were captured using a MICROfire (Olympus, Center Valley, PA) camera attached to an Olympus IX70 inverted microscope.
Roam J.L., Yan Y., Nguyen P.K., Kinstlinger I.S., Leuchter M.K., Hunter D.A., Wood M.D, & Elbert D.L. (2015). A Modular, Plasmin-Sensitive, Clickable Poly(ethylene glycol)-Heparin-Laminin Microsphere System for Establishing Growth Factor Gradients in Nerve Guidance Conduits. Biomaterials, 72, 112-124.
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