Centriprep ym 3
Centriprep YM-3 is a centrifugal filtration device used for concentrating and desalting small volume samples. It features a regenerated cellulose membrane with a molecular weight cutoff of 3,000 Daltons.
Lab products found in correlation
3 protocols using centriprep ym 3
Caco-2 Cell Culture and Supernatant Preparation
Harvesting Parasitic Worm Excretory-Secretory Proteins
Fractionation and Characterization of Sb5 CFF
Sb5 CFF was fractionated with centrifugal filter columns to estimate the sizes of active components. CFF was first loaded into Centriprep YM-3 (MWCO 3 kDa) and centrifuged as instructed by the manufacture (Millipore.com). The filtrate was designated as <3 kDa fraction. The remaining CFF in the column was subsequently loaded to YM-10 (MWCO 10 kDa) and centrifuged. The filtrate collected was designated as 3–10 kDa fraction while the remaining in the second column was processed with the third column, Centriprep YM-30. The resulting filtrate was designated as 10–30 kDa fraction and the solution that did not go through was designated as >30 kDa fraction. These four fractions were then tested together with the controls CFF and SDW for bioactivities in the microscopy and infection assays described above.
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