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7 protocols using cobalt chloride

1

Synthesis of Catalysts using Transition Metal Salts

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All the materials used for synthesizing catalysts, including ethylene glycol, poly(ethylene glycol) (PEG), sodium acetate (CH3COONa), ferric chloride (FeCl3·6H2O), manganese chloride (MnCl2·H2O), zinc chloride (ZnCl2), cupric chloride (CuCl2·2H2O), nickel chloride (NiCl2·6H2O), cobalt chloride (CoCl2·6H2O), ethyl alcohol (C2H6O), 5,5-dimethyl-1-pyrroline N-oxide (DMPO), were purchased from Sinopharm Chemical Reagent Co., Ltd. All chemicals were used without further purification.
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2

Synthesis of Nanoparticles Using Common Reagents

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Standard reagent sodium hypochlorite (NaClO, 50 mL 0.10 M) was purchased from Beijing Putian Genesis Biotechnology Co., Ltd. (Beijing, China) Tetrachloroauricacid hydrate (HAuCl4·4H2O), ascorbic acid (AA), sodium borohydride (NaBH4), silver nitrate (AgNO3), hexadecyltrimethylammoniumbromide (CTAB), ferric chloride hexahydrate, calcium chloride, magnesium sulfate, copper sulphate, aluminum nitrate, zinc nitrate, cobalt chloride, hydrochloric acid, nitric acid were purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China).
All reagents were analytical grade and without further purification. Ultrapure water was prepared with a Milli-Q water purification system (Millipore, Bedford, MA, USA).
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3

Cobalt Chloride and Graphene Oxide Synthesis

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The cobalt chloride (CoCl2, 99.5%), vitamin C (VC, 99.0%), deionized water (H2O, 99.9%), and melamine (C3H6N6, 99.8%) used in the experiments were all from Sinopharm Chemical Reagent Co., Ltd., Shanghai, China. The graphene oxide aqueous solution (GO, 2 mg/mL) was provided by Suzhou TanFeng Graphene Technology Co., Ltd., Nanjing, China. The above chemicals were used directly as raw materials without further purification.
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4

Synthesis of Colorful Metal-Organic Frameworks

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Cobalt chloride, 1,3,5-benzoic acid, sodium dodecyl benzene sulfonate, 2-methylimidazole, N,N-dimethyl bromide methyl formamide, and absolute ethanol were purchased from Sinopharm Chemical Reagent Co., Ltd. All the chemical reagents were used without further purification.
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5

Aptamer-Based Hg2+ Detection Synthesis

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Cerium nitrate hexahydrate (CeCl3·6H2O, AR), urea (AR), citric acid (CA, AR), hydrogen peroxide (H2O2, AR), potassium persulfate (K2S2O8, AR), sodium phosphate dibasic dodecahydrate (Na2HPO4·2H2O, AR), potassium dihydrogen phosphate (KH2PO4, AR), N-hydroxysuccinimide (NHS, AR), 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide (EDC, AR), and mercuric chloride (HgCl2, AR) were obtained from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Cadmium nitrate (Cd(NO3)2, AR), sodium chloride (NaCl, AR), sodium nitrate (NaNO3, AR), barium chloride (BaCl2, AR), lead nitrate (Pb(NO3)2, AR), cobalt chloride (CoCl2, AR), sodium sulfate decahydrate (Na2S·9H2O, AR), and sodium sulfate (Na2SO4, AR) were purchased from Sinopharm Chemical Reagents Co., Ltd. (Tianjin, China). The Hg2+ aptamer (5′-COOH-(CH2)6-TTTTTTTTTTTT-3′) was synthesized by Shanghai Sangon Biotechnology Co., Ltd. (Shanghai, China). The reagents used in this study were not subjected to purification treatment unless otherwise specified. The solutions involved were all prepared with ultrapure water (18.25 MΩ cm).
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6

Solvent Purification and Drying Protocol

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Sodium citrate, carbamide, methylbenzene and cobalt chloride were obtained from Sinopharm Chemical Reagent Co. Ltd. All of the reagents are analytical grade, which can be used without further purification.
Ethanol absolute (water content ≤ 0.005%, anhydrous level, E130059), tetrahydrofuran (≥99.9%, anhydrous level, T120775), and 1,4-dioxane (HPLC, ≥99.5%, D116159) were purchased from Sigma-Aldrich. All the above solvents were dried with activated 3 Å molecular sieves.
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7

Synthesis of Transition Metal Oxides

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All the reagents used were of analytical grade and used as received. Nickel chloride (NiCl2·6H2O), cobalt chloride (CoCl2·6H2O), ferric chloride (FeCl3), manganese chloride (MnCl2·4H2O), chromium chloride (CrCl3·6H2O), and glucose (C6H12O6) were purchased from Sinopharm Chemical Reagent Co. Ltd.
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