It was important to assess the change in organic acid composition of fruit juice when unprotected and γ-PGA-protected cells were added to it to determine any alteration in its nutritional and organoleptic properties. Organic acid analysis was performed using high performance liquid chromatography (HPLC), Shimadzu Class-VPV 5.03 (Kyoto, Japan). A Prevail Organic Acid 5 μm column with a UV detector was used for citric acid, malic acid and ascorbic acid. 25 mM KH2PO4 (pH 2.5 adjusted with phosphoric acid) was used as the eluent. Before loading, the samples were filtered using 0.45 μm filters and were diluted 10-fold. Standard curves were prepared using known concentrations of chemicals to be analyzed.
Class vpv 5
Manufactured by Shimadzu
Sourced in Japan
The Class-VPV 5.03 is a lab equipment product offered by Shimadzu. It serves as a versatile vacuum pump system, designed to facilitate various laboratory applications requiring a controlled vacuum environment.
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Lab products found in correlation
7 protocols using class vpv 5
1
Organic Acid Profiling in Fruit Juice
2
Xylanase Hydrolysis of Beechwood Xylan
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Fifty µL of xylanase was incubated with 1% w/v beechwood xylan in 0.05 M citrate buffer (pH 5) at 55 °C. Aliquots were withdrawn at intervals and boiled for 5 min. Control without enzyme was performed in parallel. Samples were analyzed by HPLC (HPLC, Shimadzu Class-VPV 5.03 (Kyoto, Japan) on an HPX-87H column (300 mm × 7.8 mm). The eluent was HPLC grade DI-water with a flow rate of 0.5 mL/min at 70 °C. Sugars were detected by a refractive index detector (Shodex, RI-101). Xylo-oligosaccharides (xylobiose, X2; xylotriose, X3; xylotetraose, X4; xylopentaose, X5; xylohexaose, X6) and xylose were used as standards for the analysis of the reaction products27 (link).
3
HPLC Analysis of Sugars in Filtrate
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The prepared sample was filtered through a 0.45-μm membrane. Analysis of the sugars in the filtrate was performed using HPLC (Shimadzu Class-VPV 5.03, Kyoto, Japan) equipped with a refractive index RID-10A Shimadzu detector, LC-16ADVP binary pump, DCou-14 A degasser, Shodex PL Hi-PlexPb column (Sc 1011 No. H706081), Guard column Sc-LcShodex, and heater set at 80 °C. Separation and assessment were carried out on an amino-bonded column with a mobile phase of double distilled water at a 1 mL/min flow rate. The identified peaks of the chromatographs were identified by comparing the retention times with the standards samples of galactose, glucose, fructose, sucrose, raffinose, and stachyose.
4
HPLC Analysis of Levan Extracts
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Levan sample was extracted into DMSO for HPLC analysis according to (AOAC, 1997 ). Then was filtered through a 0.45 µm cellulose nitrate Millipore membrane filter (Sartorius). Sugar in the filtrate was analyzed in the National Research Centre, Dokki, Giza, Egypt, by using HPLC, Shimadzu Class-VPV 5.03 (Kyoto, Japan) supplied with LC-16ADVP binary pump, refractive index RID-10A Shimadzu detector, DCou-14 A degasser, Shodex PL Hi-Plex Pb column (Sc 1011 No. H706081), and Sc-Lc Shodex as guard column, at 80°C. Separation and quantitation were done on an amino-bonded column with a mobile phase of CH3CN and H2O (80/20 V:V).
5
Polysaccharide Analysis of Pneumococcal Vaccines
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Samples of serotypes 19F, 6A/B and the commercial product Synflorix (10 serotypes) were filtered through a 0.45 µm membrane. Analysis of the polysaccharides in the filtrate was performed using HPLC, Shimadzu Class-VPV 5.03 equipped with refractive index RID-10A Shimadzu detector, LC-16ADVP binary pump, DCou-14 A degasser and Shodex PL Hi-PlexPb column (Sc 1011 No. H706081), Guard column Sc-LcShodex, and heater set at 65 °C. The separation and the quantitation were carried out on an amino-bonded column with a mobile phase of CH3CN and H2O (80/20 V: V) (AOAC, 1997 ; Ball, 1990 (link); Li, Andrewsw & Pehrssonw, 2002 (link)). Standard solutions of Dextran 2,000, 50,000 and 60,000–90,000 with analytical grades were prepared by diluting each concentration in 100 ml deionized water. Injection volume was 20 µl.
6
HPLC Analysis of Phytochemicals
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The extracts were analysed on Shimadzu Class-VPV 5.03 (Kyoto, Japan) equipped with Shimadzu UV-Vis detector (SPD-10Avp) at 330 nm, LC-16ADVP binary pump, DCou-14 A degasser, and Phenomenex RP-18 (UK; 250 × 4.00 mm, 5 µ) column according to Kim et al. (2006 ). The solvent gradient used in this study was formed through a solvent (A methanol and B 0.03% phosphoric acid in water). The linear gradient was used for chromatographic separation: 0 min, 70% B; 6 min, 55% B; 20 min, 45% B. The run time was 30 min. The injection volume was 5 µL. The solvent flow rate was maintained at 1.0 mL/min. All analyses were carried out at 30 °C. The spectra were recorded at 330 nm. The method of the external standard was used for quantification.
7
Quantifying Lactose Hydrolysis in Whey
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Samples were prepared according to the methods illustrated by Karkacier et al. (2003) . 20 μl of each prepared sample was injected into the HPLC (Shimadzu Class-VPV 5.03 (Kyoto, Japan) equipped with a refractive index RID-10A Shimadzu detector, LC-16ADVP binary pump and DCou-14A, Guard column Sc-LcShodex, and heater set at 80 °C). Prepare stock standard solutions for lactose, glucose, and galactose. Each sugar solution was prepared to be 2 mg ml -1 with deionized water. Each solution was stoppered and sonicated until completely dissolved. These solutions were stored in glass vials in the refrigerator (Ahmedet al., 2017) . The percentage of lactose hydrolysis is calculated as follows:
The percent lactose hydrolysis= (X -Y)/ X * 100 X= lactose content in whey before hydrolysis Y=lactose content in the whey after hydrolysis
The percent lactose hydrolysis= (X -Y)/ X * 100 X= lactose content in whey before hydrolysis Y=lactose content in the whey after hydrolysis
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