FACS analysis of mesenteric lymph node cells: CD4-APC (eBioscience, clone GK1.5), TCRb-PE (eBioscience, clone H57-597), CD69-biotin (eBioscience, clone H1.2F3), Streptavidin-FITC, (eBioscience). Immunohystochemistry: F4/80 (AbD Serotec; MCA497). Western blot: SIRT2 (H-95, SantaCruz sc-20966), Acetyl-NF-κB (Acetyl-K310, Abcam, ab19870), phospho-IKbα (ser32/36, Cell Signaling 9246), IKbα (L35A5, Cell Signaling 4814), Hsp90 (BD Transduction Laboratories, 610418).
Tcrb pe
Manufactured by Thermo Fisher Scientific
TCRb-PE is a fluorescent-conjugated antibody that detects the beta chain of the T cell receptor (TCR) complex. It is used in flow cytometry applications to identify and characterize T cell populations.
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Lab products found in correlation
Streptavidin fitc, by Thermo Fisher Scientific (1 mentions)
Cd4 apc, by Thermo Fisher Scientific (1 mentions)
F4 80, by Bio-Rad (1 mentions)
Hsp90, by BD (1 mentions)
Sirt2 h 95, by Santa Cruz Biotechnology (1 mentions)
Axiovert 200m fluorescence microscope, by Zeiss (1 mentions)
Cd44 pe, by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Fluoromount g, by Thermo Fisher Scientific (1 mentions)
2 protocols using tcrb pe
1
FACS, IHC, and Western Blot Analysis of Mesenteric Lymph Node Cells
2
Histological Analysis of Mouse Bone Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Mice were euthanized at the indicated ages, and perfused with 4% PFA. The ulna, radius, femur, and tibia of each animal were collected, placed into 4% PFA for 2 hours, and then washed with PBS. Bones were decalcified as previously described by placing the bones in 0.5M EDTA for 24 hours, then prepared for mounting by incubation in sucrose for 24 hours 29 (link) . Long bones were flash frozen in OCT (Fischer Healthcare), sectioned onto adhesive tape as previously described 30 (link) , washed, blocked with 1% rat serum in PBS, and then stained. Staining antibodies used included: DAPI, TCRb-PE (Clone: H57-597, eBioscience), and CD44-PE (Clone: IM7, eBioscience). After staining, the tissues were mounted with Fluoromount-G (Invitrogen) and imaged using a Zeiss Axiovert 200M Fluorescence Microscope (Zeiss) in the Leduc Imaging Core (Brown University). Images were analyzed with FIJI 31 (link) .
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