Pre designed probes

Manufactured by Thermo Fisher Scientific

Sourced in United States

Pre-designed probes are laboratory equipment used for targeted detection and analysis of specific genetic sequences or molecular targets. They provide a standardized and optimized solution for researchers to efficiently conduct their investigations.

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Lab products found in correlation

High capacity cdna archive kit, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Pre-designed primer/probe mixes (2 citations) Pre-designed probe sets (2 citations)

3 protocols using pre designed probes

qPCR Gene Expression Analysis

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cDNA was reverse transcribed using the High-Capacity cDNA Archive Kit (Applied Biosystems, Foster City, CA) according to the manufacturer’s protocol. qPCR was performed using pre-designed probes (Applied Biosystems), Psmb6 and Oaz1 as the normalizers, and the comparative Ct method.

Fong L.Y., Jing R., Smalley K.J., Taccioli C., Fahrmann J., Barupal D.K., Alder H., Farber J.L., Fiehn O, & Croce C.M. (2017). Integration of metabolomics, transcriptomics, and microRNA expression profiling reveals a miR-143-HK2-glucose network underlying zinc-deficiency-associated esophageal neoplasia. Oncotarget, 8(47), 81910-81925.

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Genotyping for Dental Caries Risk

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Genomic DNA was extracted from buccal cells using a QIAmp DNA isolation protocol. Twenty-four markers in ten genes (seven involved in enamel formation and three involved in immune response) were included in this study (table 1). Genotyping was performed by polymerase chain reactions using the Taqman method [Ranade et al., 2001 (link)] with an ABI PRISM® 7900HT Sequence Detection System (Foster City, CA, USA). Pre-designed probes were supplied by Applied Biosystems (Foster City, CA, USA). Markers were chosen based on previous association with caries experience, allele frequency, position on the gene, and linkage disequilibrium relationships to maximize information content.

Abbasoğlu Z., Tanboğa İ., Küchler E.C., Deeley K., Weber M., Kaspar C., Korachi M, & Vieira A.R. (2014). Early Childhood Caries is Associated with Genetic Variants in Enamel Formation and Immune Response Genes. Caries research, 49(1), 70-77.

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Reverse Transcription and qPCR Analysis

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cDNA was reverse transcribed using the High-Capacity cDNA Archive Kit (Applied Biosystems, Foster City, CA) using reverse transcription reaction volumes of 20 μl containing 1 μg of total RNA for each sample according to the manufacturer's protocol. qPCR was performed using pre-designed probes (Applied Biosystems), Psmb6 and Oaz1 as the normalizers, and the comparative Ct method.

Fong L.Y., Taccioli C., Jing R., Smalley K.J., Alder H., Jiang Y., Fadda P., Farber J.L, & Croce C.M. (2016). MicroRNA dysregulation and esophageal cancer development depend on the extent of zinc dietary deficiency. Oncotarget, 7(10), 10723-10738.

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