Cultured Cd4+T cells, lymphocytes isolated from lamina propria of the small intestine and the colon, and Peyper’s patches were labeled with PE-Cy5.5-anti-CD4 (BioLegend, San Diego, CA) and PE-anti-CD25 antibodies (BioLegend) by incubation for 1 hour at room temperature. Then, cells were fixed and permeabilized using Mouse Foxp3 Buffer Set (BD Biosences, San Jose, CA), followed by Foxp3 staining using Alexa Fluor 488 anti-Foxp3 antibody (BD Pharmingen, San Diego, CA), according to the manufacturer’s instruction. Cells were analyzed using multi-color flow cytometry to determine the percentage of positive cells using a BD LSRII system (BD Biosciences).
Pe cy5.5 anti cd4
Manufactured by BioLegend
PE-Cy5.5-anti-CD4 is a fluorescently labeled antibody that binds to the CD4 surface antigen. It is designed for use in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using pe cy5.5 anti cd4
1
Multicolor Flow Cytometry Profiling of Intestinal T Cells
2
Multicolor Flow Cytometry Profiling of Intestinal T Cells
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Cultured Cd4+T cells, lymphocytes isolated from lamina propria of the small intestine and the colon, and Peyper’s patches were labeled with PE-Cy5.5-anti-CD4 (BioLegend, San Diego, CA) and PE-anti-CD25 antibodies (BioLegend) by incubation for 1 hour at room temperature. Then, cells were fixed and permeabilized using Mouse Foxp3 Buffer Set (BD Biosences, San Jose, CA), followed by Foxp3 staining using Alexa Fluor 488 anti-Foxp3 antibody (BD Pharmingen, San Diego, CA), according to the manufacturer’s instruction. Cells were analyzed using multi-color flow cytometry to determine the percentage of positive cells using a BD LSRII system (BD Biosciences).
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