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2 protocols using apigenin 7 glucoside

1

HPLC-based Phytochemical Quantification

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Methanol HPLC grade was obtained from Panreac Química (Barcelona, Spain). Acetonitrile HPLC grade, formic acid HPLC grade, SYBR safe DNA gel stain, and the primers MatK-1RKIM-f and MatK-3FKIM-r were from Thermo Fisher Scientific (Runcorn, Cheshire, UK). Agarose MB 250 was purchased from Biotools Biotechnological and Medical Laboratories SA (Madrid, Spain). Apigenin-7-glucoside, epigallocatechin, and tiliroside were acquired from Extrasynthese (Genay Cedex, France). Acetoxy acid was from Sigma-Aldrich (St. Louis, MO, USA), and valerenic acid was from Chromadex (Irvine, CA, USA).
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2

Analytical Standards for Quantification

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All standard compounds used for chromatographic quantifications (47885-U, 2-deoxyglucose, α, β, γ, and δ tocopherols, oxalic, quinic, malic, ascorbic, citric, and fumaric acids, from Sigma-Aldrich (St. Louis, MO, USA); tocol, from Matreya (Pleasant Gap, PA, USA); chlorogenic acid, p-coumaric acid, apigenin-6-glucoside, apigenin-7-glucoside, and luteolin-6-C-glucoside, from Extrasynthèse (Genay, France); and aloin, from Alfa Aesar (Ward Hill, MA, USA)) and bioactivity assays (trolox, kojic acid, dexamethasone, ellipticine, streptomycin, and ketoconazole, from Sigma-Aldrich) had a purity level of at least 95%. All other reagents were of analytical grade and purchased from common sources.
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